ArticleOsteoarthritis and cartilage2015
Passage-dependent relationship between mesenchymal stem cell mobilization and chondrogenic potential.
Article in Osteoarthritis and cartilage, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- An Ultrasound-Responsive Bio-Adhesive Piezoelectric Hydrogel for Osteoarthritis Cartilage.Gels (Basel, Switzerland) · 2026Article
- Managing the Heterogeneity of Mesenchymal Stem Cells for Cartilage Regenerative Therapy: A Review.Bioengineering (Basel, Switzerland) · 2023Review
- Pulsed Electromagnetic Field Therapy and Direct Current Electric Field Modulation Promote the Migration of Fibroblast-like Synoviocytes to Accelerate Cartilage Repair In Vitro.Applied sciences (Basel, Switzerland) · 2022Article
- Randomized control trial of mesenchymal stem cells versus hyaluronic acid in patients with knee osteoarthritis - A Hong Kong pilot study.Journal of orthopaedic translation · 2022Article
- Heterogeneity of In Vitro Expanded Mesenchymal Stromal Cells and Strategies to Improve Their Therapeutic Actions.Pharmaceutics · 2022Review
- Biophysical Modulation of Mesenchymal Stem Cell Differentiation in the Context of Skeletal Repair.International journal of molecular sciences · 2022Review
- Congenital microtia patients: the genetically engineered exosomes released from porous gelatin methacryloyl hydrogel for downstream small RNA profiling, functional modulation of microtia chondrocytes and tissue-engineered ear cartilage regeneration.Journal of nanobiotechnology · 2022Article
- Comparative Evaluation of Synovial Multipotent Stem Cells and Meniscal Chondrocytes for Capability of Fibrocartilage Reconstruction.Cartilage · 2021Article
- Electric field stimulation for tissue engineering applications.BMC biomedical engineering · 2021Review
- Harnessing mesenchymal stem cell secretome: Effect of extracellular matrices on proangiogenic signaling.Biotechnology and bioengineering · 2020Article
- Tunneling nanotubes mediate the expression of senescence markers in mesenchymal stem/stromal cell spheroids.Stem cells (Dayton, Ohio) · 2020Article
- A Functional Tissue-Engineered Synovium Model to Study Osteoarthritis Progression and Treatment.Tissue engineering. Part A · 2019Article
- In vitro and in vivo potentialities for cartilage repair from human advanced knee osteoarthritis synovial fluid-derived mesenchymal stem cells.Stem cell research & therapy · 2018Article
- Toward understanding the role of cartilage particulates in synovial inflammation.Osteoarthritis and cartilage · 2017Article
- In-vitro chondrogenic potential of synovial stem cells and chondrocytes allocated for autologous chondrocyte implantation - a comparison : Synovial stem cells as an alternative cell source for autologous chondrocyte implantation.International orthopaedics · 2017Article
- Lectins selectively label cartilage condensations and the otic neuroepithelium within the embryonic chicken head.Journal of anatomy · 2017Article
- Human chondrocyte migration behaviour to guide the development of engineered cartilage.Journal of tissue engineering and regenerative medicine · 2017Article
- Therapeutic Benefit for Late, but Not Early, Passage Mesenchymal Stem Cells on Pain Behaviour in an Animal Model of Osteoarthritis.Stem cells international · 2017Article
- Environmental Factors That Influence Stem Cell Migration: An "Electric Field".Stem cells international · 2017Review
- Strategies to Optimize Adult Stem Cell Therapy for Tissue Regeneration.International journal of molecular sciences · 2016Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
objectiveGalvanotaxis, the migratory response of cells in response to electrical stimulation, has been implicated in development and wound healing. The use of mesenchymal stem cells (MSCs) from the synovium (synovium-derived stem cells, SDSCs) has been investigated for repair strategies. Expansion of SDSCs is necessary to achieve clinically relevant cell numbers; however, the effects of culture passage on their subsequent cartilaginous extracellular matrix production are not well understood.
methodsOver four passages of SDSCs, we measured the expression of cell surface markers (CD31, CD34, CD49c, CD73) and assessed their migratory potential in response to applied direct current (DC) electric field. Cells from each passage were also used to form micropellets to assess the degree of cartilage-like tissue formation.
resultsExpression of CD31, CD34, and CD49c remained constant throughout cell expansion; CD73 showed a transient increase through the first two passages. Correspondingly, we observed that early passage SDSCs exhibit anodal migration when subjected to applied DC electric field strength of 6 V/cm. By passage 3, CD73 expression significantly decreased; these cells exhibited cell migration toward the cathode, as previously observed for terminally differentiated chondrocytes. Only late passage cells (P4) were capable of developing cartilage-like tissue in micropellet culture.
conclusionsOur results show cell priming protocols carried out for four passages selectively differentiate stem cells to behave like chondrocytes, both in their motility response to applied electric field and their production of cartilaginous tissue.
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