Evidence map›Paper›PMID 25451981›Full record

ArticleMolecular and cellular endocrinology2015

Regulation of transcriptional activation function of rat estrogen receptor α (ERα) by novel C-terminal splice inserts.

Pallob Kundu, Min Li, Rong Lu, Enrico Stefani, Ligia Toro

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular endocrinology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Androgen and estrogen sensitivity of bird song: a comparative view on gene regulatory levels.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2018
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Pallob KunduDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA; Division of Plant Biology, Bose Institute, Kolkata 700054, India. Electronic address: pkundu@jcbose.ac.in.
Min LiDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA.
Rong LuDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA.
Enrico StefaniDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA; Department of Physiology, University of California, Los Angeles, CA 90095, USA; Department of Brain Research Institute, University of California, Los Angeles, CA 90095, USA; Department of Cardiovascular Research Laboratory, University of California, Los Angeles, CA 90095, USA.
Ligia ToroDepartment of Anesthesiology, University of California, Los Angeles, CA 90095, USA; Department of Brain Research Institute, University of California, Los Angeles, CA 90095, USA; Department of Cardiovascular Research Laboratory, University of California, Los Angeles, CA 90095, USA; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
University of California, Los Angeles · US

Funding

Revealing Cardiovascular Stress Regulation beyond the Diffraction LimitR01HL088640 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI STEFANI, ENRICO · 2007 to 2011
$5.3M
BK(Ca) channel in heart mitochondriaR01HL107418 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI OLCESE, RICCARDO, STEFANI, ENRICO · 2012 to 2015
$2.5M
NHLBI NIH HHS R01 HL088640NHLBI NIH HHS R01 HL107418
6 · The paper itself

Abstract

Estrogen receptor α (ERα) mediates estrogen diverse actions on tissues. ERα gene has eight constitutively expressing exons and is known to have multiple isoforms generated by alternative initiation of transcription and splicing events including exon skipping. We have discovered two novel exons inserted between exon 5 and 6 of rat ERα that can add independently or in tandem 18 and 14 amino acids to the estrogen binding/activator function 2 domain of the receptor. Their transcript expression is three to six fold higher in heart compared to brain, aorta, liver, ovary and uterus. In heart, the new variants increased ~2 fold with animal growth from prenatal to adulthood, and had a minor increment in aged animals (28 months). Inclusion of these exons yields a receptor with practically no binding capacity for estrogen and reduced dimerization. The new variants show nuclear localization but are less efficient in binding to estrogen responsive elements (EREs) and failed to transcriptionally activate promoters containing EREs (mSlo, KCNE2). Thus, the new variants can regulate the wild-type receptor function and may contribute to the regulatory action of estrogen, especially in the maturing heart where they are more abundant.

Indexed as

Mutagenesis, InsertionalTranscriptional ActivationAnimalsCell NucleusEstrogen Receptor alphaExonsFemaleGene Expression Regulation, DevelopmentalHEK293 CellsHeLa CellsHumansMaleMyocardiumOrgan SpecificityRatsRats, Sprague-DawleyESR1 protein, humanEstrogen Receptor alphaRNA IsoformsAlternative splicingEstrogen receptorHeartSteroid receptor coactivatorTranscription regulation

Identifiers

PMID25451981
PMCPMC4312711
OpenAlexW1985792031

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.