ArticleThe Journal of biological chemistry2015
Distinct roles of N-glycosylation at different sites of corin in cell membrane targeting and ectodomain shedding.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Hepatocyte growth factor activator inhibitor-2 rapidly inactivates airway-expressed human type II transmembrane serine proteases.The Journal of biological chemistry · 2026Article
- N-Glycosylation of AXL Receptor Tyrosine Kinase Regulates Its Stability, Phosphorylation, and Oncogenic Function.Molecular & cellular proteomics : MCP · 2026Article
- Glycosylation of canine tetherin is essential for its antiviral activity against H3N2 canine influenza virus.Frontiers in veterinary science · 2025Article
- Proprotein Convertase Subtilisin/Kexin 6 in Cardiovascular Biology and Disease.International journal of molecular sciences · 2022Review
- Structure and activity of human TMPRSS2 protease implicated in SARS-CoV-2 activation.Nature chemical biology · 2022Article
- Distribution of Cardiac and Renal Corin and Proprotein Convertase Subtilisin/Kexin-6 in the Experimental Model of Cardio-Renal Syndrome of Various Severities.Frontiers in physiology · 2021Article
- Article
- β1,4-Galactosyltransferase V Modulates Breast Cancer Stem Cells through Wnt/β-catenin Signaling Pathway.Cancer research and treatment · 2020Article
- The N-glycosylation of Equine Tetherin Affects Antiviral Activity by Regulating Its Subcellular Localization.Viruses · 2020Article
- N-Glycan-calnexin interactions in human factor VII secretion and deficiency.The international journal of biochemistry & cell biology · 2019Article
- Article
- Cross-linking, Immunoprecipitation and Proteomic Analysis to Identify Interacting Proteins in Cultured Cells.Bio-protocol · 2019Article
- Article
- Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury.PloS one · 2018Article
- Identification and functional analysis of CORIN variants in hypertensive patients.Human mutation · 2017Article
- Role of corin in the regulation of blood pressure.Current opinion in nephrology and hypertension · 2017Review
- High Hepsin expression predicts poor prognosis in Gastric Cancer.Scientific reports · 2016Article
- Advances in Understanding Hair Growth.F1000Research · 2016Review
- A novel cytoplasmic tail motif regulates mouse corin expression on the cell surface.Biochemical and biophysical research communications · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Corin is a membrane-bound protease essential for activating natriuretic peptides and regulating blood pressure. Human corin has 19 predicted N-glycosylation sites in its extracellular domains. It has been shown that N-glycans are required for corin cell surface expression and zymogen activation. It remains unknown, however, how N-glycans at different sites may regulate corin biosynthesis and processing. In this study, we examined corin mutants, in which each of the 19 predicted N-glycosylation sites was mutated individually. By Western analysis of corin proteins in cell lysate and conditioned medium from transfected HEK293 cells and HL-1 cardiomyocytes, we found that N-glycosylation at Asn-80 inhibited corin shedding in the juxtamembrane domain. Similarly, N-glycosylation at Asn-231 protected corin from autocleavage in the frizzled-1 domain. Moreover, N-glycosylation at Asn-697 in the scavenger receptor domain and at Asn-1022 in the protease domain is important for corin cell surface targeting and zymogen activation. We also found that the location of the N-glycosylation site in the protease domain was not critical. N-Glycosylation at Asn-1022 may be switched to different sites to promote corin zymogen activation. Together, our results show that N-glycans at different sites may play distinct roles in regulating the cell membrane targeting, zymogen activation, and ectodomain shedding of corin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.