ArticleThe Journal of biological chemistry2015
Characterization of a Drosophila ortholog of the Cdc7 kinase: a role for Cdc7 in endoreplication independent of Chiffon.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Profiling the host defense responses againstmLife · 2026Article
- GmCDC7 is involved in coordinating seed size and quality in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Finishing the egg.Genetics · 2024Article
- The interaction between the Dbf4 ortholog Chiffon and Gcn5 is conserved in Dipteran insect species.Insect molecular biology · 2022Article
- Coordinating transcription and replication to mitigate their conflicts in early Drosophila embryos.Cell reports · 2022Article
- Temporal control of late replication and coordination of origin firing by self-stabilizing Rif1-PP1 hubs inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos.Journal of cell science · 2022Article
- Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.G3 (Bethesda, Md.) · 2021Article
- Dbf4-Dependent Kinase: DDK-ated to post-initiation events in DNA replication.Cell cycle (Georgetown, Tex.) · 2021Review
- Genetic Basis of Increased Lifespan and Postponed Senescence inG3 (Bethesda, Md.) · 2020Article
- TheJournal of cell science · 2019Article
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cdc7 is a serine-threonine kinase that phosphorylates components of the pre-replication complex during DNA replication initiation. Cdc7 is highly conserved, and Cdc7 orthologs have been characterized in organisms ranging from yeast to humans. Cdc7 is activated specifically during late G1/S phase by binding to its regulatory subunit, Dbf4. Drosophila melanogaster contains a Dbf4 ortholog, Chiffon, which is essential for chorion amplification in Drosophila egg chambers. However, no Drosophila ortholog of Cdc7 has yet been characterized. Here, we report the functional and biochemical characterization of a Drosophila ortholog of Cdc7. Co-expression of Drosophila Cdc7 and Chiffon is able to complement a growth defect in yeast containing a temperature-sensitive Cdc7 mutant. Cdc7 and Chiffon physically interact and can be co-purified from insect cells. Cdc7 phosphorylates the known Cdc7 substrates Mcm2 and histone H3 in vitro, and Cdc7 kinase activity is stimulated by Chiffon and inhibited by the Cdc7-specific inhibitor XL413. Drosophila egg chamber follicle cells deficient for Cdc7 have a defect in two types of DNA replication, endoreplication and chorion gene amplification. However, follicle cells deficient for Chiffon have a defect in chorion gene amplification but still undergo endocycling. Our results show that Cdc7 interacts with Chiffon to form a functional Dbf4-dependent kinase complex and that Cdc7 is necessary for DNA replication in Drosophila egg chamber follicle cells. Additionally, we show that Chiffon is a member of an expanding subset of DNA replication initiation factors that are not strictly required for endoreplication in Drosophila.
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