Evidence map›Paper›PMID 25451317›Full record

ArticleCancer letters2015

LPA-mediated migration of ovarian cancer cells involves translocalization of Gαi2 to invadopodia and association with Src and β-pix.

Jeremy D Ward, Ji Hee Ha, Muralidharan Jayaraman, Danny N Dhanasekaran

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 41 citations in OpenAlex.

  1. GlialeLife · 2026
    Article
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  5. GPCRs thatSmall GTPases · 2022
    Review
  6. Article
  7. Biomolecules · 2021
    Article
  8. Review
  9. Article
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  11. Article
  12. Review
  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Jeremy D WardStephenson Cancer Center, Department of Cell Biology, The University of Oklahoma Health Sciences Center, 975 NE 10th Street, Oklahoma City, OK 73104, USA.
Ji Hee HaStephenson Cancer Center, Department of Cell Biology, The University of Oklahoma Health Sciences Center, 975 NE 10th Street, Oklahoma City, OK 73104, USA.
Muralidharan JayaramanStephenson Cancer Center, Department of Cell Biology, The University of Oklahoma Health Sciences Center, 975 NE 10th Street, Oklahoma City, OK 73104, USA.
Danny N DhanasekaranStephenson Cancer Center, Department of Cell Biology, The University of Oklahoma Health Sciences Center, 975 NE 10th Street, Oklahoma City, OK 73104, USA. Electronic address: danny-dhanasekaran@ouhsc.edu.
University of Oklahoma Health Sciences Center · US

Funding

TUMOR RESISTANCE MECHANISMS TO ANTI-VEGF THERAPY IN PROSTATE CANCER (Sukyung Woo)P20GM103639 · NIGMS · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI DHANASEKARAN, DANNY N. · 2012 to 2022
$21.3M
G Proteins and Their Receptors in Tumor Cell MetastasisR01CA116984 · NCI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI DHANASEKARAN, DANNY N. · 2007 to 2011
$1.5M
Regulation of JNK-signaling molecules by the gep oncogenesR01CA123233 · NCI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI DHANASEKARAN, DANNY N. · 2008 to 2013
$1.3M
NCI NIH HHS CA116984NCI NIH HHS CA123233NCI NIH HHS R01 CA116984NCI NIH HHS R01 CA123233NIGMS NIH HHS P20 GM103639
6 · The paper itself

Abstract

Lysophosphatidic acid (LPA) plays a critical role in the migration and invasion of ovarian cancer cells. However, the downstream spatiotemporal signaling events involving specific G protein(s) underlying this process are largely unknown. In this report, we demonstrate that LPA signaling causes the translocation of Gαi2 into the invadopodia leading to its interaction with the tyrosine kinase Src and the Rac/CDC42-specific guanine nucleotide exchange factor, β-pix. Our results establish that Gαi2 activates Rac1 through a p130Cas-dependent pathway in ovarian cancer cells. Moreover, our report reveals that knockdown of Gαi2 leads to loss of β-pix and active-Rac association in the invadopodia. We also show that knockdown of Gαi2 leads to the complete loss of translocation to p130Cas to focal adhesions. Finally, when Gαi2 is knocked down, this led to the total distribution of Src being shifted primarily from invadopodia and the leading edge of the cells to the perinuclear region, suggesting that Src is inactive in the absence of Gαi2. Overall, our report provides tantalizing evidence that Gαi2 is a critical signaling component of a large signaling complex in the invadopodia that if disrupted could serve as an excellent target for therapy in ovarian and potentially other cancers.

Indexed as

Blotting, WesternCell AdhesionCell MovementCell ProliferationCell Surface ExtensionsFemaleFocal AdhesionsGTP-Binding Protein alpha Subunit, Gi2HumansLysophospholipidsNeoplasm InvasivenessOvarian NeoplasmsProtein TransportRho Guanine Nucleotide Exchange Factorssrc-Family KinasesTumor Cells, CulturedARHGEF7 protein, humanGTP-Binding Protein alpha Subunit, Gi2lysophosphatidic acidLysophospholipidsRho Guanine Nucleotide Exchange Factorssrc-Family KinasesCell migrationG proteinGα(i2)LPAOvarian cancerSrc

Identifiers

PMID25451317
PMCPMC4821470
OpenAlexW2079613671

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.