ArticleJournal of molecular biology2015
A general computational approach for repeat protein design.
Article in Journal of molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- HURDLER: a cloning method to overcome length limits in designed repeat proteins.Nucleic acids research · 2026Article
- GeomeTRe: accurate calculation of geometrical descriptors of tandem repeat proteins.Bioinformatics (Oxford, England) · 2025Article
- Design, synthesis, and characterization of protein origami based on self-assembly of a brick and staple artificial protein pair.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Structure and stability of the designer protein WRAP-T and its permutants.Scientific reports · 2021Article
- Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering.Nature chemical biology · 2021Article
- Enhancing the promiscuity of a member of the Caspase protease family by rational design.Proteins · 2020Article
- Article
- Identification and Analysis of Natural Building Blocks for Evolution-Guided Fragment-Based Protein Design.Journal of molecular biology · 2020Article
- Modular repeat protein sculpting using rigid helical junctions.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Development and applications of artificial symmetrical proteins.Computational and structural biotechnology journal · 2020Review
- The Evolutionarily Conserved Serine Residues in BRI1 LRR Motifs Are Critical for Protein Secretion.Frontiers in plant science · 2020Article
- Ambidextrous helical nanotubes from self-assembly of designed helical hairpin motifs.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Applying graph theory to protein structures: an Atlas of coiled coils.Bioinformatics (Oxford, England) · 2018Article
- The variable lymphocyte receptor as an antibody alternative.Current opinion in biotechnology · 2018Review
- Frustration, function and folding.Current opinion in structural biology · 2018Review
- Designing cooperatively folded abiotic uni- and multimolecular helix bundles.Nature chemistry · 2018Article
- Structural Insights into VLR Fine Specificity for Blood Group Carbohydrates.Structure (London, England : 1993) · 2017Article
- ISAMBARD: an open-source computational environment for biomolecular analysis, modelling and design.Bioinformatics (Oxford, England) · 2017Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Repeat proteins have considerable potential for use as modular binding reagents or biomaterials in biomedical and nanotechnology applications. Here we describe a general computational method for building idealized repeats that integrates available family sequences and structural information with Rosetta de novo protein design calculations. Idealized designs from six different repeat families were generated and experimentally characterized; 80% of the proteins were expressed and soluble and more than 40% were folded and monomeric with high thermal stability. Crystal structures determined for members of three families are within 1Å root-mean-square deviation to the design models. The method provides a general approach for fast and reliable generation of stable modular repeat protein scaffolds.
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