Evidence map›Paper›PMID 25450855›Full record

ArticleOsteoarthritis and cartilage2015

Effects of insulin-like growth factor-1 and dexamethasone on cytokine-challenged cartilage: relevance to post-traumatic osteoarthritis.

Y Li, Y Wang, S Chubinskaya, B Schoeberl, E Florine, P Kopesky, A J Grodzinsky

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Osteoarthritis and cartilage, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed, 1 pooled it
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed, 1 synthesis or guideline pooled it, 123 citations in OpenAlex.

  1. Pooled it
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  11. Loss of collagen content is localized near cartilage lesions on the day of injurious loading and intensified on day 12.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025
    Article
  12. Article
  13. Triamcinolone acetonide has minimal effect on short- and long-term metabolic activities of cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024
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8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Y LiMassachusetts Institute of Technology, Cambridge, MA, USA.
Y WangMassachusetts Institute of Technology, Cambridge, MA, USA.
S ChubinskayaRush University Medical Center, Chicago, IL, USA.
B SchoeberlMerrimack Pharmaceuticals, USA.
E FlorineMerrimack Pharmaceuticals, USA.
P KopeskyMerrimack Pharmaceuticals, USA.
A J GrodzinskyMassachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: alg@mit.edu.
Massachusetts Institute of Technology · USMerrimack Pharmaceuticals (United States) · USRush University Medical Center · US

Funding

Cartilage Repair Using Self Assembling Peptide ScaffoldsR01AR060331 · NIAMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI GRODZINSKY, ALAN J. · 2012 to 2016
$1.7M
NIAMS NIH HHS AR60331NIAMS NIH HHS R01 AR060331
6 · The paper itself

Abstract

objectiveInterleukin-1 is one of the inflammatory cytokines elevated after traumatic joint injury that plays a critical role in mediating cartilage tissue degradation, suppressing matrix biosynthesis, and inducing chondrocyte apoptosis, events associated with progression to post-traumatic osteoarthritis (PTOA). We studied the combined use of insulin-like growth factor-1 (IGF-1) and dexamethasone (Dex) to block these multiple degradative effects of cytokine challenge to articular cartilage.

methodsYoung bovine and adult human articular cartilage explants were treated with IL-1α in the presence or absence of IGF-1, Dex, or their combination. Loss of sulfated glycosaminoglycans (sGAG) and collagen were evaluated by the DMMB and hydroxyproline assays, respectively. Matrix biosynthesis was measured via radiolabel incorporation, chondrocyte gene expression by qRT-PCR, and cell viability by fluorescence staining.

resultsIn young bovine cartilage, the combination of IGF-1 and Dex significantly inhibited the loss of sGAG and collagen, rescued the suppression of matrix biosynthesis, and inhibited the loss of chondrocyte viability caused by IL-1α treatment. In adult human cartilage, only IGF-1 rescued matrix biosynthesis and only Dex inhibited sGAG loss and improved cell viability. Thus, the combination of IGF-1 + Dex together showed combined beneficial effects in human cartilage.

conclusionsOur findings suggest that the combination of IGF-1 and Dex has greater beneficial effects than either molecule alone in preventing cytokine-mediated cartilage degradation in adult human and young bovine cartilage. Our results support the use of such a combined approach as a potential treatment relevant to early cartilage degradative changes associated with joint injury.

Indexed as

AnimalsCartilage, ArticularCattleCytokinesDexamethasoneGlucocorticoidsHumansInsulin-Like Growth Factor IInterleukin-1alphaOsteoarthritisCytokinesDexamethasoneGlucocorticoidsInsulin-Like Growth Factor IInterleukin-1alphaCartilageCytokineGlucocorticoidGrowth factorPost-traumatic osteoarthritis

Identifiers

PMID25450855
PMCPMC4304966
OpenAlexW2032108983

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.