Evidence map›Paper›PMID 25450094›Full record

ArticleJournal of dermatological science2014

Protein kinase D1 deficiency promotes differentiation in epidermal keratinocytes.

Vivek Choudhary, Lawrence O Olala, Ismail Kaddour-Djebbar, Inas Helwa, Wendy B Bollag

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dermatological science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Vivek ChoudharyCharlie Norwood VA Medical Center, Augusta, GA 30904, USA; Department of Physiology, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USA; Section of Dermatology, Department of Medicine, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USA.
Lawrence O OlalaCharlie Norwood VA Medical Center, Augusta, GA 30904, USA; Department of Physiology, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USA.
Ismail Kaddour-DjebbarCharlie Norwood VA Medical Center, Augusta, GA 30904, USA; Department of Physiology, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USA.
Inas HelwaDepartment of Oral Biology, Georgia Regents University, Augusta, GA 30912, USA; Department of Physiology, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USA.
Wendy B BollagCharlie Norwood VA Medical Center, Augusta, GA 30904, USA; Department of Physiology, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USA; Section of Dermatology, Department of Medicine, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USA; Department of Oral Biology, Georgia Regents University, Augusta, GA 30912, USA; Departments of Cell Biology and Anatomy, and Orthopaedic Surgery, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USA. Electronic address: WB@gru.edu.
Augusta University · USCharlie Norwood VA Medical Center · US

Funding

Protein Kinase D and Epidermal Structure and FunctionR21AR057321 · NIAMS · AUGUSTA UNIVERSITY · PI BOLLAG, WENDY B · 2009 to 2010
$362k
CSRD VA I01 CX000590NIAMS NIH HHS R21 AR057321NIAMS NIH HHS R21AR057321
6 · The paper itself

Abstract

backgroundProtein kinase D (PKD or PKD1) is a serine/threonine protein kinase that has been shown to play a role in a variety of cellular processes; however, the function of PKD1 in the skin has not been fully investigated. The balance between proliferation and differentiation processes in the predominant cells of the epidermis, the keratinocytes, is essential for normal skin function.

objectiveTo investigate the effect of PKD1 deficiency on proliferation and differentiation of epidermal keratinocytes.

methodsWe utilized a floxed PKD1 mouse model such that infecting epidermal keratinocytes derived from these mice with an adenovirus expressing Cre-recombinase allowed us to determine the effect of PKD1 gene loss in vitro. Proliferation and differentiation were monitored using qRT-PCR, Western blot, transglutaminase activity assays, [3H]thymidine incorporation into DNA and cell cycle analysis.

resultsA significant decrease in PKD1 mRNA and protein levels was achieved in adenoviral Cre-recombinase-infected cells. Deficiency of PKD1 resulted in significant increases in the mRNA and protein expression of various differentiation markers such as loricrin, involucrin, and keratin 10 either basally and/or upon stimulation of differentiation. PKD1-deficient keratinocytes also showed an increase in transglutaminase expression and activity, indicating an anti-differentiative role of PKD1. Furthermore, the PKD1-deficient keratinocytes exhibited decreased proliferation. However, PKD1 loss had no effect on stem cell marker expression.

conclusionsCre-recombinase-mediated knockdown represents an additional approach demonstrating that PKD1 is an anti-differentiative, pro-proliferative signal in mouse keratinocytes.

Indexed as

AnimalsCell DifferentiationCell ProliferationCells, CulturedGene Knockdown TechniquesKeratin-10KeratinocytesMembrane ProteinsMiceProtein Kinase CProtein PrecursorsRNA, MessengerTransglutaminasesUp-RegulationinvolucrinKeratin-10Krt10 protein, mouseloricrinMembrane ProteinsProtein Kinase Cprotein kinase DProtein PrecursorsRNA, Messengertransglutaminase 1TransglutaminasesDifferentiationKeratinocyteProliferationProtein kinase DTransglutaminase

Identifiers

PMID25450094
PMCPMC4259831
OpenAlexW1971674875

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.