ArticleJournal of immunological methods2014
CyTOF supports efficient detection of immune cell subsets from small samples.
Article in Journal of immunological methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
63 citing papers in PubMed.
- Activated sputum eosinophils associated with exacerbations in children on mepolizumab.The Journal of allergy and clinical immunology · 2024Trial
- Partial body cryotherapy exposure drives acute redistribution of circulating lymphocytes: preliminary findings.European journal of applied physiology · 2023Trial
- Profiling cellular heterogeneity in asthma with single cell multiparameter CyTOF.Journal of leukocyte biology · 2020Trial
- Article
- Early cellular and molecular signatures correlate with severity of West Nile virus infection.iScience · 2023Article
- Immune cell profiling in atherosclerosis: role in research and precision medicine.Nature reviews. Cardiology · 2022Review
- Depiction of immune heterogeneity of peripheral blood from patients with type II diabetic nephropathy based on mass cytometry.Frontiers in endocrinology · 2022Article
- Article
- Mass Cytometry Study on Hepatic Fibrosis and Its Drug-Induced Recovery Using Mouse Peripheral Blood Mononuclear Cells.Frontiers in immunology · 2022Article
- The role of T-lymphocytes in neuropathic pain initiation, development of chronicity and treatment.Brain, behavior, & immunity - health · 2021Review
- Single-Cell Multiomics Analysis for Drug Discovery.Metabolites · 2021Review
- Single-Cell Omics in Dissecting Immune Microenvironment of Malignant Gliomas-Challenges and Perspectives.Cells · 2021Review
- Analyzing signaling activity and function in hematopoietic cells.The Journal of experimental medicine · 2021Review
- Microglia Diversity in Healthy and Diseased Brain: Insights from Single-Cell Omics.International journal of molecular sciences · 2021Review
- Design and implementation of a prospective cohort study of persons living with and without HIV infection who are initiating medication treatment for opioid use disorder.Contemporary clinical trials communications · 2021Article
- Spatially resolved 3D metabolomic profiling in tissues.Science advances · 2021Article
- Novel Technologies in Studying Brain Immune Response.Oxidative medicine and cellular longevity · 2021Review
- Regulatory and IgEMethods in molecular biology (Clifton, N.J.) · 2021Article
- Histone deacetylase 3 controls lung alveolar macrophage development and homeostasis.Nature communications · 2020Article
- Single-cell mass cytometry on peripheral blood identifies immune cell subsets associated with primary biliary cholangitis.Scientific reports · 2020Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
Abstract
Analysis of immune cell states is paramount to our understanding of the pathogenesis of a broad range of human diseases. Immunologists rely on fluorescence cytometry for cellular analysis, and while detection of 8 markers is now well established, the overlap of fluorescent signals limits efficiency. Mass cytometry or CyTOF (Cytometry by Time-Of-Flight) is a new technology for multiparameter single cell analysis that overcomes many limitations of fluorescence-based flow cytometry and can routinely detect as many as 40 markers per sample. This technology provides tremendous detail for cellular analysis of multiple cell populations simultaneously and is a powerful technique for translational investigations. Here we present reproducible detection of immune cell subsets starting with as few as 10,000 cells. Our study provides methods to employ CyTOF for small samples, which is especially relevant for investigation of limited patient biopsies in translational and clinical research.
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