Evidence map›Paper›PMID 25448885›Full record

ArticleJournal of structural biology2015

Quantitative analysis of mouse pancreatic islet architecture by serial block-face SEM.

C R Pfeifer, A Shomorony, M A Aronova, G Zhang, T Cai, H Xu, A L Notkins, R D Leapman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 71 citations in OpenAlex.

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  15. The human α cell in health and disease.The Journal of endocrinology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

C R Pfeifer *National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20854, USA.
A Shomorony *National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20854, USA.
M A AronovaNational Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20854, USA.
G ZhangNational Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20854, USA.
T CaiNational Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20854, USA.
H XuNational Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20854, USA.
A L NotkinsNational Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20854, USA.
R D LeapmanNational Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20854, USA.
National Institutes of Health · USNational Institute of Biomedical Imaging and Bioengineering · US

Funding

Three-Dimensional Cell and Tissue Reconstruction by Serial Block Face SEMZIAEB000086 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI LEAPMAN, RICHARD · 2013 to 2025
$23.6M
Insulin-Dependent Diabetes MellitusZIADE000423 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI NOTKINS, ABNER L. · 2009 to 2020
$16.9M
Electron MicroscopyZICEB000080 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI ZHANG, GUOFENG · 2011 to 2025
$8.2M
Intramural NIH HHS ZIA EB000086
6 · The paper itself

Abstract

We have applied serial block-face scanning electron microscopy (SBF-SEM) to measure parameters that describe the architecture of pancreatic islets of Langerhans, microscopic endocrine organs that secrete insulin and glucagon for control of blood glucose. By analyzing entire mouse islets, we show that it is possible to determine (1) the distributions of alpha and beta cells, (2) the organization of blood vessels and pericapillary spaces, and (3) the ultrastructure of the individual secretory cells. Our results show that the average volume of a beta cell is nearly twice that of an alpha cell, and the total mitochondrial volume is about four times larger. In contrast, nuclear volumes in the two cell types are found to be approximately equal. Although the cores of alpha and beta secretory granules have similar diameters, the beta granules have prominent halos resulting in overall diameters that are twice those of alpha granules. Visualization of the blood vessels revealed that every secretory cell in the islet is in contact with the pericapillary space, with an average contact area of 9±5% of the cell surface area. Our data show that consistent results can be obtained by analyzing small numbers of islets. Due to the complicated architecture of pancreatic islets, such precision cannot easily be achieved by using TEM of thin sections.

Indexed as

AnimalsImaging, Three-DimensionalIslets of LangerhansMaleMiceMicroscopy, Electron, ScanningMitochondria3D reconstructionGlucagon secreting granulesInsulin secreting granulesPancreatic islets of LangerhansSerial block face SEM

Identifiers

PMID25448885
PMCPMC4305430
OpenAlexW2047641318

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.