Evidence map›Paper›PMID 25448846›Full record

ArticleMolecular and cellular endocrinology2015

Regulation of cytokine-inducible SH2-containing protein (CIS) by ubiquitination and Elongin B/C interaction.

Philip J Jensik, Lydia A Arbogast

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular endocrinology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Philip J JensikDepartment of Physiology, Southern Illinois University School of Medicine, 1135 Lincoln Drive, Carbondale, IL 62901-6523, USA.
Lydia A ArbogastDepartment of Physiology, Southern Illinois University School of Medicine, 1135 Lincoln Drive, Carbondale, IL 62901-6523, USA. Electronic address: larbogast@siumed.edu.
Southern Illinois University School of Medicine · US

Funding

Prolactin Feedback to Hypothalamic Dopaminergic NeuronsR01HD045805 · NICHD · SOUTHERN ILLINOIS UNIVERSITY CARBONDALE · PI ARBOGAST, LYDIA A · 2005 to 2009
$1.7M
Opioid-Dopamine Interactions During LactationR01HD048925 · NICHD · SOUTHERN ILLINOIS UNIVERSITY CARBONDALE · PI ARBOGAST, LYDIA A · 2005 to 2009
$1.3M
NICHD NIH HHS HD045805NICHD NIH HHS HD048925NICHD NIH HHS R01 HD045805NICHD NIH HHS R01 HD048925
6 · The paper itself

Abstract

Cytokine-inducible SH2-containing protein (CIS) inhibits prolactin receptor (PRLR) signaling and acts as part of an E3 ubiquitin ligase complex through interactions with Elongin B/C proteins. This study aimed to identify CIS lysine ubiquitination sites and determine roles of ubiquitination and Elongin B/C interactions on CIS protein stability and PRLR signaling inhibition. Site-directed mutations revealed that CIS can be ubiquitinated on all six lysine residues. Elongin B/C interaction box mutation had no influence on CIS ubiquitination. CIS stability was increased by mutation of lysine residues and further enhanced by co-mutation of Elongin B/C interaction domain. CIS inhibition of STAT5B phosphorylation and casein promoter activation was dependent on CIS interactions with Elongin B/C, but not on CIS ubiquitination. These data indicate CIS protein stability is regulated through multiple mechanisms, including ubiquitination and interaction with Elongin B/C proteins, whereas CIS functional inhibition of PRLR signaling is dependent on the Elongin B/C interaction.

Indexed as

CaseinsElonginHEK293 CellsHumansLysineMutagenesis, Site-DirectedMutationProtein StabilityReceptors, ProlactinSignal TransductionSTAT5 Transcription FactorSuppressor of Cytokine Signaling ProteinsTranscription FactorsUbiquitinationCaseinsELOB protein, humanElonginLysineReceptors, ProlactinSTAT5B protein, humanSTAT5 Transcription FactorSuppressor of Cytokine Signaling ProteinsTranscription FactorsCISProlactin receptorProtein degradationSignal transductionSTAT5Ubiquitination

Identifiers

PMID25448846
PMCPMC4373541
OpenAlexW2022191921

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.