ArticleDevelopmental biology2015
Mechanics of blastopore closure during amphibian gastrulation.
Article in Developmental biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 41 citations in OpenAlex.
- StrainMapperJ: An Easy-to-Use Digital Image Correlation Toolkit for Exploring and Quantifying the Mechanics of Deforming Tissues.Results and problems in cell differentiation · 2026Article
- Shape, Strain, and Stability: Epithelia Under High Strain.bioRxiv : the preprint server for biology · 2025Article
- The TissueTractor: A Device for Applying Large Strains to Tissues and Cells for Simultaneous High-Resolution Live Cell Microscopy.Small methods · 2025Article
- PCP-dependent polarized mechanics in the cortex of individual cells during convergent extension.Developmental biology · 2025Article
- The first embryo, the origin of cancer and animal phylogeny. V. Cancer stem cells as the unifying biomechanical principle between embryology and oncology.Mechanobiology in medicine · 2025Review
- Control of spatio-temporal patterning via cell growth in a multicellular synthetic gene circuit.Nature communications · 2024Article
- Sensing the force in living embryos.Nature materials · 2024Article
- Quantifying mechanical forces during vertebrate morphogenesis.Nature materials · 2024Article
- The TissueTractor, a device for applying large strains to tissues and cells for simultaneous high-resolution live cell microscopy.bioRxiv : the preprint server for biology · 2024Article
- A mechanochemical model recapitulates distinct vertebrate gastrulation modes.Science advances · 2023Article
- The surface ectoderm exhibits spatially heterogenous tension that correlates with YAP localisation during spinal neural tube closure in mouse embryos.Cells & development · 2023Article
- Article
- Harnessing mechanobiology for kidney organoid research.Frontiers in cell and developmental biology · 2023Review
- Chambers for Culturing and ImmobilizingCold Spring Harbor protocols · 2022Article
- Hindbrain neuropore tissue geometry determines asymmetric cell-mediated closure dynamics in mouse embryos.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Furry is required for cell movements during gastrulation and functionally interacts with NDR1.Scientific reports · 2021Article
- Convergent extension in the amphibian, Xenopus laevis.Current topics in developmental biology · 2020Review
- Adapting a Plant Tissue Model to Animal Development: Introducing Cell Sliding into VirtualLeaf.Bulletin of mathematical biology · 2019Article
- Rho kinase-dependent apical constriction counteracts M-phase apical expansion to enable mouse neural tube closure.Journal of cell science · 2019Article
- Decoupling the Roles of Cell Shape and Mechanical Stress in Orienting and Cueing Epithelial Mitosis.Cell reports · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Blastopore closure in the amphibian embryo involves large scale tissue reorganization driven by physical forces. These forces are tuned to generate sustained blastopore closure throughout the course of gastrulation. We describe the mechanics of blastopore closure at multiple scales and in different regions around the blastopore by characterizing large scale tissue deformations, cell level shape change and subcellular F-actin organization and by measuring tissue force production and structural stiffness of the blastopore during gastrulation. We find that the embryo generates a ramping magnitude of force until it reaches a peak force on the order of 0.5μN. During this time course, the embryo also stiffens 1.5 fold. Strain rate mapping of the dorsal, ventral and lateral epithelial cells proximal to the blastopore reveals changing patterns of strain rate throughout closure. Cells dorsal to the blastopore, which are fated to become neural plate ectoderm, are polarized and have straight boundaries. In contrast, cells lateral and ventral to the blastopore are less polarized and have tortuous cell boundaries. The F-actin network is organized differently in each region with the highest percentage of alignment occurring in the lateral region. Interestingly F-actin was consistently oriented toward the blastopore lip in dorsal and lateral cells, but oriented parallel to the lip in ventral regions. Cell shape and F-actin alignment analyses reveal different local mechanical environments in regions around the blastopore, which was reflected by the strain rate maps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.