Evidence map›Paper›PMID 25448112›Full record

ArticleVaccine2015

Activation of the NLRP3 inflammasome by vault nanoparticles expressing a chlamydial epitope.

Ye Zhu, Janina Jiang, Najwane Said-Sadier, Gale Boxx, Cheryl Champion, Ashley Tetlow, Valerie A Kickhoefer, Leonard H Rome, David M Ojcius, Kathleen A Kelly

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Vaccine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 24 citations in OpenAlex.

  1. NLRP3 inflammasome inhibition decreasesInfection and immunity · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Ye ZhuDepartment of Molecular Cell Biology, and Health Sciences Research Institute, University of California, Merced, CA 95343, USA.
Janina JiangDepartment of Pathology and Laboratory Medicine, University of California, Los Angeles, CA 90095, USA.
Najwane Said-SadierDepartment of Molecular Cell Biology, and Health Sciences Research Institute, University of California, Merced, CA 95343, USA.
Gale BoxxDepartment of Pathology and Laboratory Medicine, University of California, Los Angeles, CA 90095, USA.
Cheryl ChampionDepartment of Pathology and Laboratory Medicine, University of California, Los Angeles, CA 90095, USA.
Ashley TetlowDepartment of Pathology and Laboratory Medicine, University of California, Los Angeles, CA 90095, USA.
Valerie A KickhoeferDepartment of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.
Leonard H RomeDepartment of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.
David M OjciusDepartment of Molecular Cell Biology, and Health Sciences Research Institute, University of California, Merced, CA 95343, USA. Electronic address: david.ojcius@gmail.com.
Kathleen A KellyDepartment of Pathology and Laboratory Medicine, University of California, Los Angeles, CA 90095, USA. Electronic address: KKelly@mednet.ucla.edu.
University of California, Los Angeles · USUniversity of California, Merced · US

Funding

Novel ways to prevent upper GT infectionR01AI079004 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KELLY, KATHLEEN A. · 2010 to 2014
$1.9M
NIAID NIH HHS AI079004NIAID NIH HHS R01 AI079004
6 · The paper itself

Abstract

The full potential of vaccines relies on development of effective delivery systems and adjuvants and is critical for development of successful vaccine candidates. We have shown that recombinant vaults engineered to encapsulate microbial epitopes are highly stable structures and are an ideal vaccine vehicle for epitope delivery which does not require the inclusion of an adjuvant. We studied the ability of vaults which were engineered for use as a vaccine containing an immunogenic epitope of Chlamydia trachomatis, polymorphic membrane protein G (PmpG), to be internalized into human monocytes and behave as a "natural adjuvant". We here show that incubation of monocytes with the PmpG-1-vaults activates caspase-1 and stimulates IL-1β secretion through a process requiring the NLRP3 inflammasome and that cathepsin B and Syk are involved in the inflammasome activation. We also observed that the PmpG-1-vaults are internalized through a pathway that is transiently acidic and leads to destabilization of lysosomes. In addition, immunization of mice with PmpG-1-vaults induced PmpG-1 responsive CD4(+) cells upon re-stimulation with PmpG peptide in vitro, suggesting that vault vaccines can be engineered for specific adaptive immune responses. We conclude that PmpG-1-vault vaccines can stimulate NLRP3 inflammasomes and induce PmpG-specific T cell responses.

Indexed as

NanoparticlesAdjuvants, ImmunologicAnimalsBacterial Outer Membrane ProteinsBacterial VaccinesCarrier ProteinsCaspase 1Cathepsin BCD4-Positive T-LymphocytesChlamydia trachomatisEpitopesHumansInflammasomesInterleukin-1betaIntracellular Signaling Peptides and ProteinsLysosomesAdjuvants, ImmunologicBacterial Outer Membrane ProteinsBacterial VaccinesCarrier ProteinsCaspase 1Cathepsin BEpitopesInflammasomesInterleukin-1betaIntracellular Signaling Peptides and ProteinsNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mousePmpG protein, Chlamydia trachomatisProtein-Tyrosine KinasesSyk KinaseSYK protein, humanSyk protein, mouseChlamydiaInflammasomesLysosomesVaults

Identifiers

PMID25448112
PMCPMC4272900
OpenAlexW1979504652

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.