ArticleActa tropica2015
Application of real-time PCR for the detection of Strongyloides spp. in clinical samples in a reference center in Spain.
Article in Acta tropica, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 2 of them syntheses that pooled it.
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Who cites it
32 citing papers in PubMed, 2 syntheses or guidelines pooled it, 67 citations in OpenAlex.
- Systematic review of the prevalence of Gastrointestinal helminths in ruminants in Mexico.Veterinary research communications · 2025Pooled it
- Pooled it
- Co-infection prevalence of helminths and associated risk factors among schoolchildren in the Lake Tana Basin, Northwest Ethiopia.BMC infectious diseases · 2025Article
- ChronicJournal of medical microbiology · 2024Article
- Concomitant Serological and Molecular Methods forTropical medicine and infectious disease · 2024Article
- A 14-year review (2007-2020) of helminthiasis epidemiology in a hospital in Southern Madrid, Spain.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Review
- Prevalence and public health relevance of enteric parasites in domestic dogs and cats in the region of Madrid (Spain) with an emphasis on Giardia duodenalis and Cryptosporidium sp.Veterinary medicine and science · 2023Article
- A fatal case of Strongyloides hyperinfection with obscure risk factors.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- From past to present: opportunities and trends in the molecular detection and diagnosis of Strongyloides stercoralis.Parasites & vectors · 2023Review
- Prevalence of Strongyloides stercoralis infection and associated clinical symptoms among schoolchildren living in different altitudes of Amhara National Regional State, northwest Ethiopia.PLoS neglected tropical diseases · 2022Article
- Evaluation of five diagnostic methods for Strongyloides stercoralis infection in Amhara National Regional State, northwest Ethiopia.BMC infectious diseases · 2022Article
- Effectiveness and Safety of a Single-Dose Ivermectin Treatment for Uncomplicated Strongyloidiasis in Immunosuppressed Patients (ImmunoStrong Study): The Study Protocol.Pathogens (Basel, Switzerland) · 2021Article
- Strongyloides stercoralis disseminated infection in an HIV-infected adult.PLoS neglected tropical diseases · 2020Article
- Strongyloidiasis in Southern Alicante (Spain): Comparative Retrospective Study of Autochthonous and Imported Cases.Pathogens (Basel, Switzerland) · 2020Article
- Asymptomatic Strongyloidiasis among Latin American Migrants in Spain: A Community-Based Approach.Pathogens (Basel, Switzerland) · 2020Article
- Protist enteroparasites in wild boar (Sus scrofa ferus) and black Iberian pig (Sus scrofa domesticus) in southern Spain: a protective effect on hepatitis E acquisition?Parasites & vectors · 2020Article
- Epidemiology of intestinal helminthiases in a rural community of Ethiopia: Is it time to expand control programs to include Strongyloides stercoralis and the entire community?PLoS neglected tropical diseases · 2020Article
- Lateral Flow Dipstick Test for Serodiagnosis of Strongyloidiasis.The American journal of tropical medicine and hygiene · 2019Article
- Comparison of Loop-Mediated Isothermal Amplification and Real-Time PCR Assays for Detection of Strongyloides Larvae in Different Specimen Matrices.Journal of clinical microbiology · 2019Article
- Diagnostic comparison of Baermann funnel, Koga agar plate culture and polymerase chain reaction for detection of human Strongyloides stercoralis infection in Maluku, Indonesia.Parasitology research · 2018Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strongyloidiasis is one of the major intestinal helminthic infections in humans with a worldwide distribution, affecting especially tropical and subtropical regions. This disease can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection. Definitive diagnosis of Strongyloides stercoralis infection relies mainly on demonstration of larvae in stool, but at present there is no gold standard for this diagnosis. Our main objective was to evaluate a real-time PCR targeting the 18S rRNA gene of Strongyloides spp. and to compare it with routine parasitological methods. DNA from Strongyloides venezuelensis was used to optimize PCR protocols obtaining an analytical sensitivity of 0.1 pg of parasite DNA per sample. Sensitivity and specificity of real-time PCR on fecal samples from 231 patients screened for suspected strongyloidiasis attending two hospitals in Madrid were 93.8% and 86.5%, respectively. No significant differences were found when comparing Ct-values of positive PCR between parasitological positive and negative samples. This study showed that real-time PCR is an effective tool for diagnosing strongyloidiasis and could be applied in association with parasitological methods in epidemiological studies in endemic areas. It would be also important to assess its performance in immunocompromised populations who are at risk of fatal disease.
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