Evidence map›Paper›PMID 25447462›Full record

ArticleAnalytical biochemistry2015

Method for measuring lipid mediators, proteins, and messenger RNAs from a single tissue specimen.

Jessica A Cottrell, Hsuan-Ni Lin, J Patrick O'Connor

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Vanadium salt inhibits osteoclast formation and preserves bone integrity in a rat model of type-1 diabetes associated bone loss.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026
    Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jessica A CottrellDepartment of Biochemistry and Molecular Biology, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ 07103, USA.
Hsuan-Ni LinDepartment of Biochemistry and Molecular Biology, Graduate School of Biomedical Sciences, Rutgers, The State University of New Jersey, Newark, NJ 07103, USA.
J Patrick O'ConnorDepartment of Biochemistry and Molecular Biology, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ 07103, USA; Department of Biochemistry and Molecular Biology, Graduate School of Biomedical Sciences, Rutgers, The State University of New Jersey, Newark, NJ 07103, USA. Electronic address: oconnojp@njms.rutgers.edu.

Funding

Local Modulation of Inflammation to Heal Cranial-facial Bone DefectsR01DE019926 · NIDCR · UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL · PI O'CONNOR, J PATRICK, RICCI, JOHN · 2009 to 2012
$2.5M
NIDCR NIH HHS R01 DE019926NIDCR NIH HHS R01DE019926
6 · The paper itself

Abstract

This article describes a new method for extracting RNA, protein, and lipid mediators from a single tissue specimen. Specifically, mouse bone fracture callus specimens were extracted into a single solution that was processed using three different procedures to measure messenger RNA (mRNA) levels by reverse transcription-quantitative polymerase chain reaction (RTqPCR), cytokines and growth factors using an xMAP method, and lipid mediators by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This method has several advantages because it decreases the number of animals necessary for experimentation, allows division of the sample from a homogeneous mixture that reduces sample variability, and uses a solution that protects the integrity of the macromolecules during storage.

Indexed as

Reverse Transcriptase Polymerase Chain ReactionAnimalsBony CallusChromatography, High Pressure LiquidCytokinesFractures, BoneIntercellular Signaling Peptides and ProteinsLipidsMiceRNA, MessengerSolid Phase ExtractionTandem Mass SpectrometryCytokinesIntercellular Signaling Peptides and ProteinsLipidsRNA, MessengerBone regenerationFracture healingLipid mediatorMetabolomics

Identifiers

PMID25447462
PMCPMC4257868

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.