Evidence map›Paper›PMID 25447046›Full record

ArticleThe American journal of pathology2015

Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis.

Ke Yuan, Mark E Orcholski, Cristina Panaroni, Eric M Shuffle, Ngan F Huang, Xinguo Jiang, Wen Tian, Eszter K Vladar, Lingli Wang, Mark R Nicolls and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.

0numbers the graph read from it
0cells of the map it votes in
55citing papers in PubMed
4.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

55 citing papers in PubMed, 79 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Pericytes and Lung Vascular Remodeling.Arteriosclerosis, thrombosis, and vascular biology · 2026
    Review
  5. Article
  6. Article
  7. Signaling pathways and targeted therapy for pulmonary hypertension.Signal transduction and targeted therapy · 2025
    Review
  8. Review
  9. Deciphering the Complexities of Pulmonary Hypertension: The Emergent Role of Single-Cell Omics.American journal of respiratory cell and molecular biology · 2025
    Review
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Biology and function of pericytes in the vascular microcirculation.Animal models and experimental medicine · 2023
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Ke YuanDivision of Pulmonary and Critical Care Medicine, Stanford University, Stanford, California; Stanford Cardiovascular Institute, Stanford University, Stanford, California.
Mark E OrcholskiDivision of Pulmonary and Critical Care Medicine, Stanford University, Stanford, California; Stanford Cardiovascular Institute, Stanford University, Stanford, California.
Cristina PanaroniDivision of Endocrinology, Gerontology & Metabolism, Stanford University, Stanford, California.
Eric M ShuffleDivision of Pulmonary and Critical Care Medicine, Stanford University, Stanford, California; Stanford Cardiovascular Institute, Stanford University, Stanford, California.
Ngan F HuangStanford Cardiovascular Institute, Stanford University, Stanford, California; VA Palo Alto Health Care System, Stanford University, Stanford, California; Department of Cardiothoracic Surgery, Stanford University, Stanford, California.
Xinguo JiangStanford Cardiovascular Institute, Stanford University, Stanford, California; VA Palo Alto Health Care System, Stanford University, Stanford, California.
Wen TianStanford Cardiovascular Institute, Stanford University, Stanford, California; VA Palo Alto Health Care System, Stanford University, Stanford, California.
Eszter K VladarDepartment of Pathology, Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford University School of Medicine, Stanford, California.
Lingli WangDepartment of Pediatrics, Stanford University School of Medicine, Stanford, California.
Mark R NicollsStanford Cardiovascular Institute, Stanford University, Stanford, California; VA Palo Alto Health Care System, Stanford University, Stanford, California.
Joy Y WuDivision of Endocrinology, Gerontology & Metabolism, Stanford University, Stanford, California.
Vinicio A de Jesus PerezDivision of Pulmonary and Critical Care Medicine, Stanford University, Stanford, California; Stanford Cardiovascular Institute, Stanford University, Stanford, California. Electronic address: vdejesus@stanford.edu.
Cardiovascular Institute of the South · USStanford University · USVA Palo Alto Health Care System · US

Funding

Critical Role for Microvasculature in Airway TransplantationR01HL095686 · NHLBI · PALO ALTO VETERANS INSTIT FOR RESEARCH · PI NICOLLS, MARK ROBERT · 2010 to 2022
$4.7M
The role of the Wnt/planar cell polarity pathway in pulmonary angiogenesisK08HL105884 · NHLBI · STANFORD UNIVERSITY · PI DE JESUS PEREZ, VINICIO A · 2012 to 2016
$701k
NHLBI NIH HHS K08 HL105884NHLBI NIH HHS R01 HL095686
6 · The paper itself

Abstract

Pericytes are perivascular cells localized to capillaries that promote vessel maturation, and their absence can contribute to vessel loss. Whether impaired endothelial-pericyte interaction contributes to small vessel loss in pulmonary arterial hypertension (PAH) is unclear. Using 3G5-specific, immunoglobulin G-coated magnetic beads, we isolated pericytes from the lungs of healthy subjects and PAH patients, followed by lineage validation. PAH pericytes seeded with healthy pulmonary microvascular endothelial cells failed to associate with endothelial tubes, resulting in smaller vascular networks compared to those with healthy pericytes. After the demonstration of abnormal polarization toward endothelium via live-imaging and wound-healing studies, we screened PAH pericytes for abnormalities in the Wnt/planar cell polarity (PCP) pathway, which has been shown to regulate cell motility and polarity in the pulmonary vasculature. PAH pericytes had reduced expression of frizzled 7 (Fzd7) and cdc42, genes crucial for Wnt/PCP activation. With simultaneous knockdown of Fzd7 and cdc42 in healthy pericytes in vitro and in a murine model of angiogenesis, motility and polarization toward pulmonary microvascular endothelial cells were reduced, whereas with restoration of both genes in PAH pericytes, endothelial-pericyte association was improved, with larger vascular networks. These studies suggest that the motility and polarity of pericytes during pulmonary angiogenesis are regulated by Wnt/PCP activation, which can be targeted to prevent vessel loss in PAH.

Indexed as

Cell PolarityNeovascularization, PathologicAdolescentAdultAnimalscdc42 GTP-Binding ProteinCell MovementChildCoculture TechniquesDisease Models, AnimalEndothelial CellsFemaleFrizzled ReceptorsGene Knockdown TechniquesHumansHypertension, Pulmonarycdc42 GTP-Binding ProteinCdc42 protein, mouseFrizzled ReceptorsFzd7 protein, mouseImmunoglobulin GReceptors, G-Protein-CoupledRNA, Small InterferingWnt Proteins

Identifiers

PMID25447046
PMCPMC4278244
OpenAlexW2080448840

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.