ArticleJournal of clinical pathology1989
Interphase cytogenetics using biotin and digoxigenin labelled probes II: Simultaneous differential detection of human and papilloma virus nucleic acids in individual nuclei.
Article in Journal of clinical pathology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 55 citations in OpenAlex.
- Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.The Histochemical journal · 1995Review
- Discrimination of closely homologous human genomic and viral sequences in cells and tissues: further characterization of Tmt.The Histochemical journal · 1994Article
- The discrimination of high-risk HPV types by in situ hybridization and the polymerase chain reaction.The Histochemical journal · 1993Article
- Nonradioactive in situ hybridization using digoxigenin-labeled oligonucleotides. Applications to musculoskeletal tissues.The American journal of pathology · 1992Article
- Detection of high risk human papillomavirus in routine cervical smears: strategy for screening.Journal of clinical pathology · 1992Article
- Rapid detection of hepatitis B virus DNA in liver tissue by in situ hybridisation and its combination with immunohistochemistry for simultaneous detection of HBV antigens.Journal of clinical pathology · 1991Article
- Model system for optimising mRNA non-isotopic in situ hybridisation: riboprobe detection of lysozyme mRNA in archival gut biopsy specimens.Journal of clinical pathology · 1991Article
- In situ hybridisation in perspective.Journal of clinical pathology · 1991Review
- Detection of mRNA and hnRNA using a digoxigenin labelled cDNA probe by in situ hybridization on frozen tissue sections.The Histochemical journal · 1991Article
- Interphase cytogenetics using biotin and digoxigenin labelled probes: III. Increased sensitivity and flexibility for detecting HPV in cervical biopsy specimens and cell lines.Journal of clinical pathology · 1991Article
- Discrimination of closely homologous HPV types by nonisotopic in situ hybridization: definition and derivation of tissue melting temperatures.The Histochemical journal · 1990Article
- In situ hybridisation with digoxigenin-labelled DNA probes for detection of viral genomes.Journal of clinical pathology · 1990Article
- Detection of messenger RNA using a digoxigenin end labelled oligodeoxynucleotide probe.Journal of clinical pathology · 1990Article
- Interphase cytogenetics.Neurochemical research · 1990Review
- Interphase cytogenetics using biotin and digoxigenin labelled probes I: relative sensitivity of both reporter molecules for detection of HPV16 in CaSki cells.Journal of clinical pathology · 1989Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method was developed for the simultaneous detection of viral and human DNA in contrasting colours in routine formalin fixed, paraffin wax embedded biopsy specimens. This was achieved by non-isotopic in situ hybridisation (NISH) with a biotinylated Y chromosome probe and digoxigenin labelled probe for human papilloma virus type 6 (HPV 6). The tissues studied were peripheral lymphocytes, tonsil, and penile warts. The hybridisation signals produced by biotinylated probes were visualised in red using streptavidin peroxidase and those produced by digoxigenin labelled probes as a blue/black colour using anti-digoxigenin alkaline phosphatase. In lymphocytes and tonsil 95-100% of cells had a detectable Y chromosome; in warts only 60-70% of infected keratinocytes near the skin surface had a demonstrable Y chromosome. This suggests that this chromosome is lost or occluded in cell maturation. In simultaneous double hybridisation with both probes, HPV and Y sequences were demonstrable within the same nucleus in penile warts. This technique permits the simultaneous differential detection of two nuclei acid sequences in interphase nuclei and will have application in analysis of putative dual HPV infections and in determining the intranuclear spatial relations between nucleic acids in interphase nuclei.
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