ArticleJournal of clinical pathology1989
Interphase cytogenetics using biotin and digoxigenin labelled probes I: relative sensitivity of both reporter molecules for detection of HPV16 in CaSki cells.
Article in Journal of clinical pathology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 87 citations in OpenAlex.
- Comparative analysis of human papillomavirus detection by PCR and non-isotopic in situ hybridisation.Journal of clinical pathology · 1995Trial
- Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Next-generation in situ hybridization approaches to define and quantify HIV and SIV reservoirs in tissue microenvironments.Retrovirology · 2018Review
- Localisation of HHV-8 in AIDS related lymphadenopathy.Molecular pathology : MP · 2000Article
- Identification of HHV8 in early Kaposi's sarcoma: implications for Kaposi's sarcoma pathogenesis.Molecular pathology : MP · 1998Article
- Detection of Cytochrome P450 mRNA in Tissue Sections and Cell Lines Using Enzyme-Labeled Fluorescence In Situ Hybridization.In vitro toxicology · 1997Article
- A one-day double-labelling technique for tissue specimens: immunogold-silver staining for in situ hybridization combined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemistry for antigens.The Histochemical journal · 1996Article
- Detection of human herpesvirus 6 and human papillomavirus 16 in cervical carcinoma.The American journal of pathology · 1994Article
- Kinetic microphotometric evaluation of in situ hybridization for mRNA of slow myosin heavy chain in type I and C fibres of rabbit muscle.Histochemistry · 1994Article
- Discrimination of closely homologous human genomic and viral sequences in cells and tissues: further characterization of Tmt.The Histochemical journal · 1994Article
- Article
- Expression of p53 protein in laryngeal squamous cell carcinoma and dysplasia: possible correlation with human papillomavirus infection and clinicopathological findings.Virchows Archiv : an international journal of pathology · 1994Review
- The discrimination of high-risk HPV types by in situ hybridization and the polymerase chain reaction.The Histochemical journal · 1993Article
- Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation.Journal of clinical pathology · 1992Article
- Nonradioactive in situ hybridization using digoxigenin-labeled oligonucleotides. Applications to musculoskeletal tissues.The American journal of pathology · 1992Article
- Detection of high risk human papillomavirus in routine cervical smears: strategy for screening.Journal of clinical pathology · 1992Article
- Detection of human papillomavirus in matched cervical smears and biopsy specimens by non-isotopic in situ hybridisation.Journal of clinical pathology · 1992Article
- In situ hybridisation of albumin mRNA in normal liver and hepatocellular carcinoma with a digoxigenin labelled oligonucleotide probe.Journal of clinical pathology · 1992Article
- Episomal and integrated human papillomavirus in cervical neoplasia shown by non-isotopic in situ hybridisation.Journal of clinical pathology · 1991Article
- Rapid detection of hepatitis B virus DNA in liver tissue by in situ hybridisation and its combination with immunohistochemistry for simultaneous detection of HBV antigens.Journal of clinical pathology · 1991Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was undertaken to develop technology for the detection of nucleic acid using two different DNA probe reporter molecules, the ultimate aim being to differentially label two nucleic acids within the same nucleus. Digoxigenin and biotin were used to label DNA probes. The absolute and relative sensitivity of digoxigenin and biotin labelled DNA probes for detecting integrated human papilloma virus 16 (HPV16) was investigated in CaSki cells by non-isotopic in situ hybridisation (NISH). Several methods for the detection of labelled probes were also investigated. The optimal sensitivity of digoxigenin labelled probe was equivalent to that of biotin when alkaline phosphatase was used as the final detector. The median number of discrete viral signals discernible in each cell with the most sensitive detection system was seven to eight with both labelled probes. The average number of HPV16 genomes in each CaSki cell, derived by dot blot hybridisation, was about 270. The calculated absolute sensitivity of NISH for viral detection in this system is complex because of variation of signal size and number. Nevertheless, one signal per nucleus equates to as little as 30 to 40 viral copies, and probably much less. The ability to distinguish up to 15 discrete signals with both digoxigenin and biotin labelled probes in the nuclei of CaSki cells indicates that these methods will be useful in interphase cytogenetics in material routinely fixed in aldehyde.
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