Evidence map›Paper›PMID 25442009›Full record

ArticleJournal of biomechanics2014

Promoting increased mechanical properties of tissue engineered neocartilage via the application of hyperosmolarity and 4α-phorbol 12,13-didecanoate (4αPDD).

Jennifer K Lee, Courtney A Gegg, Jerry C Hu, Philip H Kass, Kyriacos A Athanasiou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomechanics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jennifer K LeeDepartment of Biomedical Engineering, University of California, One Shields Avenue, Davis, CA 95616, USA.
Courtney A GeggDepartment of Biomedical Engineering, University of California, One Shields Avenue, Davis, CA 95616, USA.
Jerry C HuDepartment of Biomedical Engineering, University of California, One Shields Avenue, Davis, CA 95616, USA.
Philip H KassDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California, 1089 Veterinary Medicine Drive, Davis, CA 95616, USA.
Kyriacos A AthanasiouDepartment of Biomedical Engineering, University of California, One Shields Avenue, Davis, CA 95616, USA; Department of Orthopaedic Surgery, University of California, One Shields Avenue, Davis, CA 95616, USA. Electronic address: athanasiou@ucdavis.edu.

Funding

Engineering Lubrication in Tissue Engineered CartilageR01AR061496 · NIAMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ATHANASIOU, KYRIACOS A, REDDI, AKEPATI HARI · 2011 to 2014
$1.3M
NIAMS NIH HHS R01 AR061496NIAMS NIH HHS R01AR061496NIGMS NIH HHS T32-GM00799
6 · The paper itself

Abstract

Osteoarthritis, a degenerative disease of the load-bearing joints, greatly reduces quality of life for millions of Americans and places a tremendous cost on the American healthcare system. Due to limitations of current treatments, tissue engineering of articular cartilage may provide a promising therapeutic option to treat cartilage defects. However, cartilage tissue engineering has yet to recapitulate the functional properties of native tissue. During normal joint loading, cartilage tissue experiences variations in osmolarity and subsequent changes in ionic concentrations. Motivated by these known variations in the cellular microenvironment, this study sought to improve the mechanical properties of neocartilage constructs via the application of hyperosmolarity and transient receptor potential vanilloid 4 (TRPV4) channel activator 4α-phorbol 12,13-didecanoate (4αPDD). It was shown that 4αPDD elicited significant increases in compressive properties. Importantly, when combined, 4αPDD positively interacted with hyperosmolarity to modulate its effects on tensile stiffness and collagen content. Thus, this study supports 4αPDD-activated channel TRPV4 as a purported mechanosensor and osmosensor that can facilitate the cell and tissue level responses to improve the mechanical properties of engineered cartilage. To our knowledge, this study is the first to systematically evaluate the roles of hyperosmolarity and 4αPDD on the functional (i.e., mechanical and biochemical) properties of self-assembled neotissue. Future work may combine 4αPDD-induced channel activation with other chemical and mechanical stimuli to create robust neocartilages suitable for treatment of articular cartilage defects.

Indexed as

Tissue EngineeringAnimalsBiomechanical PhenomenaCartilage, ArticularCattleCells, CulturedChondrocytesCollagenOsmolar ConcentrationPhorbol EstersTRPV Cation ChannelsCollagenphorbol-12,13-didecanoatePhorbol EstersTRPV Cation ChannelsCartilage tissue engineeringDynamic loadingNeocartilageScaffold-freeSelf-assembly

Identifiers

PMID25442009
PMCPMC5107315

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.