ArticleMalaria journal2014
Changes in metabolic phenotypes of Plasmodium falciparum in vitro cultures during gametocyte development.
Article in Malaria journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Degradation of ribosomal RNA duringmBio · 2025Article
- Degradation of ribosomal RNA duringbioRxiv : the preprint server for biology · 2025Article
- Induction of gametocytogenesis in human malaria parasites: from stress to genome editing.Frontiers in microbiology · 2025Review
- Genetic complexity alters drug susceptibility of asexual and gametocyte stages ofAntimicrobial agents and chemotherapy · 2024Article
- Molecular Mechanisms of Persistence in Protozoan Parasites.Microorganisms · 2023Review
- TheAntimicrobial agents and chemotherapy · 2023Article
- Adapt or Die: Targeting Unique Transmission-Stage Biology for Malaria Elimination.Frontiers in cellular and infection microbiology · 2022Review
- Lactic Acid Supplementation Increases Quantity and Quality of Gametocytes in Plasmodium falciparum Culture.Infection and immunity · 2020Article
- Structural, Functional, and Metabolic Alterations in Human Cerebrovascular Endothelial Cells duringMicroorganisms · 2020Article
- Novel Method for the Separation of Male and Female Gametocytes of the Malaria ParasitemSphere · 2020Article
- From Metabolite to Metabolome: Metabolomics Applications inFrontiers in microbiology · 2020Review
- In vitro Multistage Malaria Transmission Blocking Activity of Selected Malaria Box Compounds.Drug design, development and therapy · 2020Article
- Hierarchical transcriptional control regulates Plasmodium falciparum sexual differentiation.BMC genomics · 2019Article
- Polyunsaturated fatty acids promoteBiology open · 2019Article
- tRNA epitranscriptomics and biased codon are linked to proteome expression inMolecular systems biology · 2018Article
- Developing transmission-blocking strategies for malaria control.PLoS pathogens · 2017Review
- Quantitative chromatin proteomics reveals a dynamic histone post-translational modification landscape that defines asexual and sexual Plasmodium falciparum parasites.Scientific reports · 2017Article
- Sex-Specific Biology of the Human Malaria Parasite Revealed from the Proteomes of Mature Male and Female Gametocytes.Molecular & cellular proteomics : MCP · 2017Article
- The transcriptome of the mosquito Aedes fluviatilis (Diptera: Culicidae), and transcriptional changes associated with its native Wolbachia infection.BMC genomics · 2017Article
- Biological characterization of chemically diverse compounds targeting the Plasmodium falciparum coenzyme A synthesis pathway.Parasites & vectors · 2016Article
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4 authors.
Funding
Abstract
backgroundGametocytes are the Plasmodium life stage that is solely responsible for malaria transmission. Despite their important role in perpetuating malaria, gametocyte differentiation and development is poorly understood.
methodsTo shed light on the biochemical changes that occur during asexual and gametocyte development, metabolic characterization of media from in vitro intra-erythrocytic Plasmodium falciparum cultures was performed throughout gametocyte development by applying 1H nuclear magnetic spectroscopy, and using sham erythrocyte cultures as controls. Spectral differences between parasite and sham cultures were assessed via principal component analyses and partial-least squares analyses, and univariate statistical methods.
resultsClear parasite-associated changes in metabolism were observed throughout the culture period, revealing differences between asexual parasites and gametocyte stages. With culture progression and development of gametocytes, parasitic release of the glycolytic end products lactate, pyruvate, alanine, and glycerol, were found to be dramatically reduced whilst acetate release was greatly increased. Also, uptake of lipid moieties CH(2), CH(3), and CH = CH-CH(2)-CH(2) increased throughout gametocyte development, peaking with maturity.
conclusionsThis study uniquely presents an initial characterization of the metabolic exchange between parasite and culture medium during in vitro P. falciparum gametocyte culture. Results suggest that energy metabolism and lipid utilization between the asexual stages and gametocytes is different. This study provides new insights for gametocyte-specific nutritional requirements to aid future optimization and standardization of in vitro gametocyte cultivation, and highlights areas of novel gametocyte cell biology that deserve to be studied in greater detail and may yield new targets for transmission-blocking drugs.
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