ArticleFertility and sterility2015
Granulocyte colony-stimulating factor prevents loss of spermatogenesis after sterilizing busulfan chemotherapy.
Article in Fertility and sterility, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02783937 (Filgrastim for Treatment of Premature Ovarian Insufficiency), which is not on this map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Filgrastim for Treatment of Premature Ovarian Insufficiency: Randomized Clinical Trial
Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Effect of Granulocyte Colony-Stimulating Factor on the Development of Spermatogenesis in the Adulthood of Juvenile AML Mice Model Treated with Cytarabine.International journal of molecular sciences · 2023Article
- Lovastatin promotes the self-renewal of murine and primate spermatogonial stem cells.Stem cell reports · 2023Article
- Granulocyte-colony stimulating factor does not prevent in vitro cisplatin-induced germ cell reduction in immature human and mouse testis.BMC cancer · 2023Article
- Granulocyte Colony-Stimulating Factor Restored Impaired Spermatogenesis and Fertility in an AML-Chemotherapy Mice Model.International journal of molecular sciences · 2021Article
- Approaches and Technologies in Male Fertility Preservation.International journal of molecular sciences · 2020Review
- Exposure to Chemotherapy During Childhood or Adulthood and Consequences on Spermatogenesis and Male Fertility.International journal of molecular sciences · 2020Review
- Bisamide Derivative of Dicarboxylic Acid Contributes to Restoration of Testicular Tissue Function and Influences Spermatogonial Stem Cells in Metabolic Disorders.Frontiers in cell and developmental biology · 2020Article
- Oncofertility: Pharmacological Protection and Immature Testicular Tissue (ITT)-Based Strategies for Prepubertal and Adolescent Male Cancer Patients.International journal of molecular sciences · 2019Review
- Differential role of r-met-hu G-CSF on male reproductive function and development in prepubertal domestic mammals.PloS one · 2019Article
- Cancer treatment in childhood and testicular function: the importance of the somatic environment.Endocrine connections · 2018Review
- Granulocyte colony-stimulating factor (G-CSF) promotes spermatogenic regeneration from surviving spermatogonia after high-dose alkylating chemotherapy.Reproductive biology and endocrinology : RB&E · 2017Article
- Cytokines in Male Fertility and Reproductive Pathologies: Immunoregulation and Beyond.Frontiers in endocrinology · 2017Review
- Seminiferous epithelium damage after short period of busulphan treatment in adult rats and vitamin BInternational journal of experimental pathology · 2016Article
- The regulatory repertoire of PLZF and SALL4 in undifferentiated spermatogonia.Development (Cambridge, England) · 2016Article
- The Magea gene cluster regulates male germ cell apoptosis without affecting the fertility in mice.Scientific reports · 2016Article
- Increased expression of dermatopontin and its implications for testicular dysfunction in mice.Molecular medicine reports · 2016Article
- Adult Human Mesenchymal Stem Cell Differentiation at the Cell Population and Single-Cell Levels Under Alternating Electric Current.Tissue engineering. Part C, Methods · 2016Article
- Effect of human recombinant granulocyte colony-stimulating factor on rat busulfan-induced testis injury.Journal of molecular histology · 2016Article
- Histological Study on the Protective Effect of Endogenous Stem Cell Mobilization in Busulfan-Induced Testicular Injury in Albino Rats.Journal of microscopy and ultrastructureArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectiveTo determine whether granulocyte colony-stimulating factor (G-CSF) could prevent loss of spermatogenesis induced by busulfan chemotherapy via protection of undifferentiated spermatogonia, which might serve as an adjuvant approach to preserving male fertility among cancer patients.
designLaboratory animal study.
settingUniversity. ANIMAL(S): Laboratory mice. INTERVENTION(S): Five-week-old mice were treated with a sterilizing busulfan dose and with 7 days of G-CSF or vehicle treatment and evaluated 10 weeks later (experiment 1) or 24 hours after treatment (experiment 2). MAIN OUTCOME MEASURE(S): Experiment 1: testis weights, epididymal sperm counts, testis histology. Experiment 2: PLZF immunofluorescent costaining with apoptotic markers. Molecular analysis of G-CSF receptor expression in undifferentiated spermatogonia. RESULT(S): Ten weeks after treatment, busulfan-treated mice that also received treatment with G-CSF exhibited significantly better recovery of spermatogenesis and epididymal sperm counts than animals receiving busulfan alone. G-CSF led to increased numbers of PLZF+ spermatogonia 24 hours after treatment that was not accompanied by changes in apoptosis. To address the cellular target of G-CSF, mRNA for the G-CSF receptor, Csf3r, was found in adult mouse testes and cultured THY1+ (undifferentiated) spermatogonia, and cell-surface localized CSF3R was observed on 3% of cultured THY1+ spermatogonia. CONCLUSION(S): These results demonstrate that G-CSF protects spermatogenesis from gonadotoxic insult (busulfan) in rodents, and this may occur via direct action on CSF3R+ undifferentiated spermatogonia. G-CSF treatment might be an effective adjuvant therapy to preserve male fertility in cancer patients receiving sterilizing treatments.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.