Evidence map›Paper›PMID 25439840›Full record

Trial reportFertility and sterility2015

Intrafollicular cortisol levels inversely correlate with cumulus cell lipid content as a possible energy source during oocyte meiotic resumption in women undergoing ovarian stimulation for in vitro fertilization.

Ariel A Simerman, David L Hill, Tristan R Grogan, David Elashoff, Nigel J Clarke, Ellen H Goldstein, Alexa N Manrriquez, Gregorio D Chazenbalk, Daniel A Dumesic

Abstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Fertility and sterility, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 2 pooled it
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 2 syntheses or guidelines pooled it, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Ariel A SimermanDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California.
David L HillART Reproductive Center, Beverly Hills, California.
Tristan R GroganDepartment of Medicine Statistics Core, University of California, Los Angeles, Los Angeles, California.
David ElashoffDepartment of Medicine Statistics Core, University of California, Los Angeles, Los Angeles, California.
Nigel J ClarkeQuest Diagnostics Nichols Institute, San Juan Capistrano, California.
Ellen H GoldsteinDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California.
Alexa N ManrriquezDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California.
Gregorio D ChazenbalkDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California.
Daniel A DumesicDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California. Electronic address: ddumesic@mednet.ucla.edu.
University of California, Los Angeles · USART Reproductive Center · USQuest Diagnostics (United States) · US

Funding

UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
NCATS NIH HHS UL1 TR000124NCATS NIH HHS UL1TR000124
6 · The paper itself

Abstract

objectiveTo determine whether follicular fluid (FF) cortisol levels affect cumulus cell (CC) lipid content during oocyte meiotic resumption, and whether CCs express genes for glucocorticoid action.

designProspective cohort study.

settingAcademic medical center. PATIENT(S): Thirty-seven nonobese women underwent ovarian stimulation for in vitro fertilization (IVF). INTERVENTION(S): At oocyte retrieval, FF was aspirated from the first follicle (>16 mm in size) of each ovary and pooled CCs were collected. MAIN OUTCOME MEASURE(S): Follicular fluid cortisol and cortisone analysis was performed with the use of liquid chromatography-tandem mass spectrometry. CCs were stained with lipid fluorescent dye Bodipy FL C16 to determine lipid content with the use of confocal microscopy. Quantitative real-time polymerase chain reaction was used to detect CC gene expression of 11β-hydroxysteroid dehydrogenase (11β-HSD) types 1 and 2, glucocorticoid receptor (NR3C1), lipoprotein lipase (LPL), and hormone-sensitive lipase (HSL). RESULT(S): Adjusting for maternal age, FF cortisol levels negatively correlated with CC lipid content and positively correlated with numbers of total and mature oocytes. CCs expressed genes for 11β-HSD type 1 as the predominant 11β-HSD isoform, NR3C1, LPL, and HSL. CONCLUSION(S): FF cortisol levels may regulate CC lipolysis during oocyte meiotic resumption and affect oocyte quality during IVF.

Indexed as

Lipid MetabolismMeiosisOvulation InductionAdultCumulus CellsEnergy MetabolismFemaleHumansHydrocortisoneMiddle AgedOocytesOvarian FollicleStatistics as TopicHydrocortisoneCortisolcumulus cell lipidin vitro fertilizationmeiosisoocyte developmental competence

Identifiers

PMID25439840
PMCPMC4380141
OpenAlexW2085988752

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.