Observational studyOphthalmology2015
Identification of torque teno virus in culture-negative endophthalmitis by representational deep DNA sequencing.
Observational study in Ophthalmology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 123 citations in OpenAlex.
- Virus in infectious uveitis: bibliometric analysis and a clinical study.Frontiers in microbiology · 2025Pooled it
- Determinants of Outcomes of Adenoviral Keratoconjunctivitis.Ophthalmology · 2018Trial
- Paucibacterial Microbiome and Resident DNA Virome of the Healthy Conjunctiva.Investigative ophthalmology & visual science · 2016Trial
- Visual Acuity Influences Visual Outcome in Endophthalmitis: A 10-Year Retrospective Observational Cohort Analysis.Ophthalmology and therapy · 2026Article
- Association of Seasonal Hyperacute Panuveitis Syndrome withOphthalmology science · 2026Article
- Metagenomic next-generation sequencing: a game-changer in the diagnosis of unique intraocular infections.Eye (London, England) · 2025Article
- Treatment patterns, outcomes and its associated factors among seasonal hyper-acute panuveitis cases in one of the largest outbreaks: a hospital-based retrospective study from Nepal.BMC ophthalmology · 2025Article
- Overview of Microorganisms: Bacterial Microbiome, Mycobiome, Virome Identified Using Next-Generation Sequencing, and Their Application to Ophthalmic Diseases.Microorganisms · 2025Review
- The diagnosis and treatment progress of infectious endophthalmitis.Eye (London, England) · 2025Review
- Nanopore targeted sequencing identifies pathogens in patients with postoperative endophthalmitis.International journal of ophthalmology · 2025Article
- Trends and hot spots in research related to aqueous humor from 2014 to 2023: A bibliometric analysis.Heliyon · 2024Article
- A clinical metagenomic study of biopsies from Mexican endophthalmitis patients reveals the presence of complex bacterial communities and a diversity of resistance genes.Access microbiology · 2024Article
- The diagnostic utility of nanopore targeted sequencing in suspected endophthalmitis.International ophthalmology · 2023Article
- Investigating the Ocular Surface Microbiome: What Can It Tell Us?Clinical ophthalmology (Auckland, N.Z.) · 2023Review
- Systemic prime exacerbates the ocular immune response to heat-killed Mycobacterium tuberculosis.Experimental eye research · 2022Article
- Deep Metagenomic Sequencing for Endophthalmitis Pathogen Detection Using a Nanopore Platform.American journal of ophthalmology · 2022Article
- Application Progress of High-Throughput Sequencing in Ocular Diseases.Journal of clinical medicine · 2022Review
- Molecular Diagnostics for Ocular Infectious Diseases: LXXVIII Edward Jackson Memorial Lecture.American journal of ophthalmology · 2022Review
- Low-dose radiation treatment for painful plantar enthesophyte: a highly effective therapy with little side effects.European journal of medical research · 2022Article
- Application of High-Throughput Sequencing Technology in Identifying the Pathogens in Endophthalmitis.Journal of ophthalmology · 2022Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
purposeTo test the hypothesis that uncultured organisms may be present in cases of culture-negative endophthalmitis by use of deep DNA sequencing of vitreous biopsies.
designSingle-center, consecutive, prospective, observational study.
participantsAqueous or vitreous biopsies from 21 consecutive patients presenting with presumed infectious endophthalmitis and 7 vitreous samples from patients undergoing surgery for noninfectious retinal disorders.
methodsTraditional bacterial and fungal culture, 16S quantitative polymerase chain reaction (qPCR), and a representational deep-sequencing method (biome representational in silico karyotyping [BRiSK]) were applied in parallel to samples to identify DNA sequences corresponding to potential pathogens.
main outcome measuresPresence of potential pathogen DNA in ocular samples.
resultsZero of 7 control eyes undergoing routine vitreous surgery yielded positive results for bacteria or virus by culture or 16S polymerase chain reaction (PCR). A total of 14 of the 21 samples (66.7%) from eyes harboring suspected infectious endophthalmitis were culture-positive, the most common being Staphylococcal and Streptococcal species. There was good agreement among culture, 16S bacterial PCR, and BRiSK methodologies for culture-positive cases (Fleiss' kappa of 0.621). 16S PCR did not yield a recognizable pathogen sequence in any culture-negative sample, whereas BRiSK suggested the presence of Streptococcus in 1 culture-negative sample. With the use of BRiSK, 57.1% of culture-positive and 100% of culture-negative samples demonstrated the presence of torque teno virus (TTV) sequences, compared with none in the controls (P=0.0005, Fisher exact test). The presence of TTV viral DNA was confirmed in 7 cases by qPCR. No other known viruses or potential pathogens were identified in these samples.
conclusionsCulture, 16S qPCR, and BRiSK provide complementary information in presumed infectious endophthalmitis. The majority of culture-negative endophthalmitis samples did not contain significant levels of bacterial DNA. "Culture negativity" does not seem to be due to failure of growth of fastidious bacteria. The small DNA virus TTV was unexpectedly found in all culture-negative samples and some culture-positive samples. This study cannot distinguish whether TTV is a direct intraocular pathogen, an adjuvant for inflammation, a general marker of inflammation, or a commensal virus but provides a testable hypothesis for a pathogenic mechanism in culture-negative endophthalmitis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.