ArticlePathogens (Basel, Switzerland)2014
Role of daptomycin in the induction and persistence of the viable but non-culturable state of Staphylococcus aureus biofilms.
Article in Pathogens (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
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- Exploring diflunisal as a synergistic agent againstFrontiers in microbiology · 2024Article
- Induction of Viable but Non-Culturable State in Clinically Relevant Staphylococci and Their Detection with Bacteriophage K.Antibiotics (Basel, Switzerland) · 2023Article
- Nanoemulsion as an Effective Inhibitor of Biofilm-forming Bacterial Associated Drug Resistance: An Insight into COVID Based Nosocomial Infections.Biotechnology and bioprocess engineering : BBE · 2022Review
- Harnessing ultrasound-stimulated phase change contrast agents to improve antibiotic efficacy against methicillin-resistantBiofilm · 2021Article
- Contribution of Drugs Interfering with Protein and Cell Wall Synthesis to the Persistence ofInternational journal of molecular sciences · 2021Article
- Think Twice before Prescribing Antibiotics for That Swollen Knee: The Influence of Antibiotics on the Diagnosis of Periprosthetic Joint Infection.Antibiotics (Basel, Switzerland) · 2021Review
- Selective digestive decontamination solution used as "lock therapy" prevents and eradicates bacterial biofilm in an in vitro bench-top model.Annals of clinical microbiology and antimicrobials · 2020Article
- Study on the Viable but Non-culturable (VBNC) State Formation ofFrontiers in microbiology · 2020Article
- Review
- A Fast and Easy ATP-Based Approach Enables MIC Testing for Non-resuscitating VBNC Pathogens.Frontiers in microbiology · 2019Article
- Relationship between the Viable but Nonculturable State and Antibiotic Persister Cells.Journal of bacteriology · 2018Review
- Development of a New Application for Comprehensive Viability Analysis Based on Microbiome Analysis by Next-Generation Sequencing: Insights into Staphylococcal Carriage in Human Nasal Cavities.Applied and environmental microbiology · 2018Article
- Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens.Frontiers in microbiology · 2017Review
- New Insight into Daptomycin Bioavailability and Localization in Staphylococcus aureus Biofilms by Dynamic Fluorescence Imaging.Antimicrobial agents and chemotherapy · 2016Article
- Anti-biofilm Activity as a Health Issue.Frontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently demonstrated that antibiotic pressure can induce the viable but non-culturable (VBNC) state in Staphylococcus aureus biofilms. Since dormant bacterial cells can undermine anti-infective therapy, a greater understanding of the role of antibiotics of last resort, including daptomycin, is crucial. Methicillin-resistant S. aureus 10850 biofilms were maintained on non-nutrient (NN) agar in the presence or absence of the MIC of daptomycin until loss of culturability. Viable cells were monitored by epifluorescence microscopy and flow cytometry for 150 days. All biofilms reached non-culturability at 40 days and showed a similar amount of viable cells; however, in biofilms exposed to daptomycin, their number remained unchanged throughout the experiment, whereas in those maintained on NN agar alone, no viable cells were detected after 150 days. Gene expression assays showed that after achievement of non-culturability, 16S rDNA and mecA were expressed by all biofilms, whereas glt expression was found only in daptomycin-exposed biofilms. Our findings suggest that low daptomycin concentrations, such as those that are likely to obtain within biofilms, can influence the viability and gene expression of non-culturable S. aureus cells. Resuscitation experiments are needed to establish the VBNC state of daptomycin-exposed biofilms.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.