ArticleCellular reprogramming2014
MicroRNA-34c expression in donor cells influences the early development of somatic cell nuclear transfer bovine embryos.
Article in Cellular reprogramming, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- PRAMEY enhances sperm-egg binding and modulates epigenetic dynamics in bovine embryogenesis.Cell and tissue research · 2025Article
- Epididymal-Born circRNA Cargo and Its Implications in Male Fertility.International journal of molecular sciences · 2025Review
- Sperm-borne small non-coding RNAs: potential functions and mechanisms as epigenetic carriers.Cell & bioscience · 2025Review
- Article
- From follicle to blastocyst: microRNA-34c from follicular fluid-derived extracellular vesicles modulates blastocyst quality.Journal of animal science and biotechnology · 2024Article
- Sperm-borne microRNA-34c regulates maternal mRNA degradation and preimplantation embryonic development in mice.Reproductive biology and endocrinology : RB&E · 2023Article
- Correlation between Sperm Micro Ribonucleic Acid-34b and -34c Levels and Clinical Outcomes of Intracytoplasmic Sperm Injection in Men with Male Factor Infertility.International journal of molecular sciences · 2022Article
- Rebuttal from Nickole Moon, Christopher Morgan and Tracy L. Bale.The Journal of physiology · 2022Article
- MiR-34b/c play a role in early sex differentiation of Amur sturgeon, Acipenser schrenckii.Frontiers in zoology · 2022Article
- New Wenshen Shengjing Decoction Improves Early Embryonic Development by Maintaining Low Levels of H3K4me3 in Sperm.BioMed research international · 2022Article
- Association of microRNAs With Embryo Development and Fertilization in Women Undergoing Subfertility Treatments: A Pilot Study.Frontiers in reproductive health · 2021Article
- The Role of the Epididymis and the Contribution of Epididymosomes to Mammalian Reproduction.International journal of molecular sciences · 2020Review
- MicroRNA expression profile analysis in sperm reveals hsa-mir-191 as an auspicious omen of in vitro fertilization.BMC genomics · 2020Article
- Sperm-borne miR-449b influences cleavage, epigenetic reprogramming and apoptosis of SCNT embryos in bovine.Scientific reports · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The essence of the reprogramming activity of somatic cell nuclear transfer (SCNT) embryos is to produce normal fertilized embryos. However, reprogramming of somatic cells is not as efficient as the reprogramming of sperm. In this report, we describe the effect of an inducible, specific miR-34 microRNA expression in donor cells that enables a similar level of sperm:transgene expression on the early development of SCNT embryos. Our results showed that donor cells with doxycycline (dox)-induced miR-34c expression for the preparation of SCNT embryos resulted in altered developmental rates, histone modification (H3K9ac and H3K4me3), and extent of apoptosis. The cleavage rate and blastocyst formation of the induced nuclear transfer (NT) group were significantly increased. The immunofluorescence signal of H3K9ac in embryos in the induced NT group significantly increased in two-cell- and eight-cell-stage embryos; that of H3K4me3 increased significantly in eight-cell-stage embryos. Although significant differences in staining signals of apoptosis were not detected between groups, lower apoptosis levels were observed in the induced NT group. In conclusion, miR-34c expression induced by dox treatment enhances the developmental potential of SCNT embryos, modifies the epigenetic status, and changes blastocyst quality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.