ArticlePathogens (Basel, Switzerland)2014
Application of DNA Aptamers and Quantum Dots to Lateral Flow Test Strips for Detection of Foodborne Pathogens with Improved Sensitivity versus Colloidal Gold.
Article in Pathogens (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 180 citations in OpenAlex.
- Preliminary Development of a Hybrid Aptamer and Antibody Fluorescent Lateral Flow Assay for Ultrasensitive Detection of Tau441 Protein in Serum: Toward Point-of-Care Diagnostics for Alzheimer's Disease.Journal of fluorescence · 2026Article
- Ultrasensitive Staphylococcal Enterotoxin B Quantum Dot-Based Lateral Flow Immunoassays for Rapid Testing of Poultry Rinsates and Milk.Journal of fluorescence · 2025Article
- Preliminary Development of DNA Aptamer Quantum Dot-Based Competitive Lateral Flow Assays for Saxitoxin and Tetrodotoxin.Journal of fluorescence · 2025Article
- Development of a Protein-Free Nucleic Acid Lateral Flow Assay forAnalytical chemistry · 2025Article
- The Development Potential of AuNPs-Based Lateral Flow Technology Combined with Other Advanced Technologies in POCT.Applied biochemistry and biotechnology · 2025Review
- Flower-Shaped PCR Scaffold-Based Lateral Flow Bioassay forInternational journal of molecular sciences · 2024Article
- Lateral flow assays: Progress and evolution of recent trends in point-of-care applications.Materials today. Bio · 2024Review
- Mesoporous Silica Nanoparticles-Enhanced Microarray Technology for Highly Sensitive Simultaneous Detection of Multiplex Foodborne Pathogens.ACS applied bio materials · 2024Article
- Protein-Based Anchoring Methods for Nucleic Acid Detection in Lateral Flow Format Assays.Micromachines · 2023Article
- Recent Advances in Quantum Dot-Based Lateral Flow Immunoassays for the Rapid, Point-of-Care Diagnosis of COVID-19.Biosensors · 2023Review
- An In Situ Study on Nanozyme Performance to Optimize Nanozyme-Strip for Aβ Detection.Sensors (Basel, Switzerland) · 2023Article
- Recent Advances on Peptide-Based Biosensors and Electronic Noses for Foodborne Pathogen Detection.Biosensors · 2023Review
- New Advances in Lateral Flow Immunoassay (LFI) Technology for Food Safety Detection.Molecules (Basel, Switzerland) · 2022Review
- Quantum Dot-Based Lateral Flow Immunoassay as Point-of-Care Testing for Infectious Diseases: A Narrative Review of Its Principle and Performance.Diagnostics (Basel, Switzerland) · 2022Review
- Recent Advances in Silica-Nanomaterial-Assisted Lateral Flow Assay.Bioengineering (Basel, Switzerland) · 2022Review
- Applications in Which Aptamers Are Needed or Wanted in Diagnostics and Therapeutics.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Vancomycin-Loaded Furriness Amino Magnetic Nanospheres for Rapid Detection of Gram-Positive Water Bacterial Contamination.Nanomaterials (Basel, Switzerland) · 2022Article
- Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays.Sensors and actuators. B, Chemical · 2022Article
- Aptamer Conjugated Indium Phosphide Quantum Dots with a Zinc Sulphide Shell as Photoluminescent Labels forNanomaterials (Basel, Switzerland) · 2021Article
- Recent advances in gold nanoparticle-based lateral flow immunoassay for the detection of bacterial infection.Archives of microbiology · 2021Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Preliminary studies aimed at improving the sensitivity of foodborne pathogen detection via lateral flow (LF) test strips by use of high affinity DNA aptamers for capture and reporter functions when coupled to red-emitting quantum dots (Qdot 655) are reported. A variety of DNA aptamers developed against Escherichia coli, Listeria monocytogenes, and Salmonella enterica were paired in capture and reporter combinations to determine which yielded the strongest detection of their cognate bacteria using a colloidal gold screening system. Several promising sandwich combinations were identified for each of the three bacterial LF strip systems. The best E. coli aptamer-LF system was further studied and yielded a visible limit of detection (LOD) of ~3,000 E. coli 8739 and ~6,000 E. coli O157:H7 in buffer. These LODs were reduced to ~300-600 bacterial cells per test respectively by switching to a Qdot 655 aptamer-LF system. Novel aspects of these assays such as the use of high levels of detergents to avoid quantum dot agglutination and enhance migration in analytical membranes, identification of optimal analytical membrane types, UV-immobilization of capture aptamers, and novel dual biotin/digoxigenin-end labeled aptamer streptavidin-colloidal gold or -Qdot 655 conjugates plus anti-digoxigenin antibody control lines are also discussed. In general, this work provides proof-of-principle for highly sensitive aptamer-Qdot LF strip assays for rapid foodborne pathogen detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.