ArticleCells2014
Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays.
Article in Cells, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- T cell immunomonitoring: a comparative analysis of traditional and novel methods to quantify and characterize human antigen-specific T cells.Nature protocols · 2026Review
- Article
- Deep learning enabled label-free microfluidic droplet classification for single cell functional assays.Frontiers in bioengineering and biotechnology · 2024Article
- Evaluation of Cellular Immune Response to Adeno-Associated Virus-Based Gene Therapy.The AAPS journal · 2023Review
- Updated Insights into the T Cell-Mediated Immune Response against SARS-CoV-2: A Step towards Efficient and Reliable Vaccines.Vaccines · 2023Review
- In situ 3D spatiotemporal measurement of soluble biomarkers in spheroid culture.In vitro models · 2022Article
- T cell immunity is key to the pandemic endgame: How to measure and monitor it.Current research in immunology · 2022Review
- Rapid development of analytical methods for evaluating pandemic vaccines: a COVID-19 perspective.Bioanalysis · 2021Article
- Vaccine-Induced Cellular Immunity againstVaccines · 2021Review
- Utility of interferon gamma/tumor necrosis factor alpha FluoroSpot assay in differentiation between active tuberculosis and latent tuberculosis infection: a pilot study.BMC infectious diseases · 2021Article
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Tumor-Induced Inflammatory Cytokines and the Emerging Diagnostic Devices for Cancer Detection and Prognosis.Frontiers in oncology · 2021Review
- Single-Cell Technologies for the Study of Antibody-Secreting Cells.Frontiers in immunology · 2021Review
- Localized Cell-Surface Sampling of a Secreted Factor Using Cell-Targeting Beads.Analytical chemistry · 2020Article
- ELISPOT assays and their diagnostic potential in Lyme disease and Lyme neuroborreliosis.Clinical and experimental immunology · 2020Article
- Human Cytomegalovirus (HCMV)-Specific CD4Journal of virology · 2017Article
- The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity.Journal of visualized experiments : JoVE · 2016Article
- Validation of an IFNγ/IL2 FluoroSpot assay for clinical trial monitoring.Journal of translational medicine · 2016Article
- Guidelines for the automated evaluation of Elispot assays.Nature protocols · 2015Article
- Immune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay.Journal for immunotherapy of cancer · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ELISpot is one of the most commonly used immune monitoring assays, which allows the functional assessment of the immune system at the single cell level. With its outstanding sensitivity and ease of performance, the assay has recently advanced from the mere single function cell analysis to multifunctional analysis by implementing detection reagents that are labeled with fluorophores (FluoroSpot), allowing the detection of secretion patterns of two or more analytes in a single well. However, the automated evaluation of such assays presents various challenges for image analysis. Here we dissect the technical and methodological requirements for a reliable analysis of FluoroSpot assays, introduce important quality control measures and provide advice for proper interpretation of results obtained by automated imaging systems.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.