ArticlePloS one2014
Different regions of the newcastle disease virus fusion protein modulate pathogenicity.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Insights into the multifunctionality of viral glycoproteins F and HN in the lifecycle and pathogenesis of Newcastle disease virus: a systematic review.Veterinary research · 2025Pooled it
- Detection of PPMV-1 antibodies in pigeons using a blocking ELISA based on an NP-specific monoclonal antibody.Poultry science · 2026Article
- Molecular characterization of Newcastle disease virus (AOAV-1) obtained from Western region of Libya.Open veterinary journal · 2024Article
- Newcastle disease virus: the past and current situation in Indonesia.Journal of veterinary science · 2024Review
- Introduction of a Newcastle disease virus challenge strain (sub-genotype VII.1.1) isolated in Iran.Veterinary research forum : an international quarterly journal · 2023Article
- Newcastle disease burden in Nepal and efficacy of Tablet I2 vaccine in commercial and backyard poultry production.PloS one · 2023Article
- Putative Novel Avian Paramyxovirus (AMPV) and Reidentification of APMV-2 and APMV-6 to the Species Level Based on Wild Bird Surveillance (United States, 2016-2018).Applied and environmental microbiology · 2022Article
- Surveillance of Class I Newcastle Disease Virus at Live Bird Markets in China and Identification of Variants with Increased Virulence and Replication Capacity.Journal of virology · 2022Article
- Fatal encephalitis caused by Newcastle disease virus in a child.Acta neuropathologica · 2021Article
- Biological Characterization and Evolutionary Dynamics of Pigeon Paramyxovirus Type 1 in China.Frontiers in veterinary science · 2021Article
- Clinical, molecular and cytopathological characterization of a Newcastle disease virus from an outbreak in Baghdad, Iraq.Veterinary medicine and science · 2020Article
- Review
- Application of Newcastle disease virus in the treatment of colorectal cancer.World journal of clinical cases · 2019Review
- Molecular characterization of hemagglutinin-neuraminidase fragment gene of Newcastle disease virus isolated from periodically-vaccinated farms.Veterinary world · 2018Review
- Avian Respiratory Coinfection and Impact on Avian Influenza Pathogenicity in Domestic Poultry: Field and Experimental Findings.Veterinary sciences · 2018Review
- Two mutations in the HR2 region of Newcastle disease virus fusion protein with a cleavage motif "RRQRRL" are critical for fusogenic activity.Virology journal · 2017Article
- Detection of specific antigens of Newcastle disease virus using an absorbed Western blotting method.Iranian journal of veterinary research · 2017Article
- Different Origins of Newcastle Disease Virus Hemagglutinin-Neuraminidase Protein Modulate the Replication Efficiency and Pathogenicity of the Virus.Frontiers in microbiology · 2017Article
- A Review of Functional Motifs Utilized by Viruses.Proteomes · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Newcastle disease virus (NDV), also designated as Avian paramyxovirus type 1 (APMV-1), is the causative agent of a notifiable disease of poultry but it exhibits different pathogenicity dependent on the virus strain. The molecular basis for this variability is not fully understood. The efficiency of activation of the fusion protein (F) is determined by presence or absence of a polybasic amino acid sequence at an internal proteolytic cleavage site which is a major determinant of NDV virulence. However, other determinants of pathogenicity must exist since APMV-1 of high (velogenic), intermediate (mesogenic) and low (lentogenic) virulence specify a polybasic F cleavage site. We aimed at elucidation of additional virulence determinants by constructing a recombinant virus that consists of a lentogenic NDV Clone 30 backbone and the F protein gene from a mesogenic pigeon paramyxovirus-1 (PPMV-1) isolate with an intracerebral pathogenicity index (ICPI) of 1.1 specifying the polybasic sequence R-R-K-K-R*F motif at the cleavage site. The resulting virus was characterized by an ICPI of 0.6, indicating a lentogenic pathotype. In contrast, alteration of the cleavage site G-R-Q-G-R*L of the lentogenic Clone 30 to R-R-K-K-R*F resulted in a recombinant virus with an ICPI of 1.36 which was higher than that of parental PPMV-1. Substitution of different regions of the F protein of Clone 30 by those of PPMV-1, while maintaining the polybasic amino acid sequence at the F cleavage site, resulted in recombinant viruses with ICPIs ranging from 0.59 to 1.36 suggesting that virulence is modulated by regions of the F protein other than the polybasic cleavage site.
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