ArticlePloS one2014
Tissue engineering of rat bladder using marrow-derived mesenchymal stem cells and bladder acellular matrix.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Tracheal Regeneration: Recent Progress in the Application of Stem Cells in Tracheal Bioengineering.International journal of molecular sciences · 2026Review
- A Triple-Layered Composite Scaffold of Silk Fibroin and Decellularized Amniotic Membrane for Bladder Tissue Engineering.Macromolecular bioscience · 2025Article
- Recent advances and future directions in urinary system tissue engineering.Materials today. Bio · 2025Review
- Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods.Frontiers in bioengineering and biotechnology · 2022Review
- The Significance of Biomechanics and Scaffold Structure for Bladder Tissue Engineering.International journal of molecular sciences · 2021Review
- Recellularization of Native Tissue Derived Acellular Scaffolds with Mesenchymal Stem Cells.Cells · 2021Review
- TP63 basal cells are indispensable during endoderm differentiation into proximal airway cells on acellular lung scaffolds.NPJ Regenerative medicine · 2021Article
- Current Applications and Future Directions of Bioengineering Approaches for Bladder Augmentation and Reconstruction.Frontiers in surgery · 2021Review
- [Advance in functional bladder engineering].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2020Review
- A Lipid-Nanosphere-Small MyoD Activating RNA-Bladder Acellular Matrix Graft Scaffold [NP(saMyoD)/BAMG] Facilitates Rat Injured Bladder Muscle Repair and Regeneration [NP(saMyoD)/BAMG].Frontiers in pharmacology · 2020Article
- Mesenchymal stromal cells modulate the molecular pattern of healing process in tissue-engineered urinary bladder: the microarray data.Stem cell research & therapy · 2019Article
- Understanding the role of mesenchymal stem cells in urinary bladder regeneration-a preclinical study on a porcine model.Stem cell research & therapy · 2018Article
- Bioengineering Approaches for Bladder Regeneration.International journal of molecular sciences · 2018Review
- Bladder biomechanics and the use of scaffolds for regenerative medicine in the urinary bladder.Nature reviews. Urology · 2018Review
- The impact of immune response on endochondral bone regeneration.NPJ Regenerative medicine · 2018Review
- Does the Mesenchymal Stem Cell Source Influence Smooth Muscle Regeneration in Tissue-Engineered Urinary Bladders?Cell transplantation · 2017Article
- Stem Cells in Functional Bladder Engineering.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2016Review
- Decellularized Extracellular Matrix as an In Vitro Model to Study the Comprehensive Roles of the ECM in Stem Cell Differentiation.Stem cells international · 2016Review
- Potential of Newborn and Adult Stem Cells for the Production of Vascular Constructs Using the Living Tissue Sheet Approach.BioMed research international · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bladder replacement or augmentation is required in congenital malformations or following trauma or cancer. The current surgical solution involves enterocystoplasty but is associated with high complication rates. Strategies for bladder tissue engineering are thus actively sought to address this unmet clinical need. Because of the poor efficacy of synthetic polymers, the use of bladder acellular matrix (BAM) has been proposed. Indeed when cellular components are removed from xenogenic or allogeneic bladders, the extracellular matrix scaffold thus obtained can be used alone or in combination with stem cells. In this study, we propose the use of BAM seeded with marrow-derived mesenchymal stem cells (MSCs) for bladder tissue engineering. We optimized a protocol for decellularization of bladder tissue from different species including rat, rabbit and swine. We demonstrate the use of non-ionic detergents followed by nuclease digestion results in efficient decellularization while preserving the extracellular matrix. When MSCs were seeded on acellular matrix scaffold, they remained viable and proliferative while adopting a cellular phenotype consistent with their microenvironment. Upon transplantation in rats after partial cystectomy, MSC-seeded BAM proved superior to unseeded BAM with animals recovering nearly 100% normal bladder capacity for up to six months. Histological analyses also demonstrated increased muscle regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.