ArticlePathogens (Basel, Switzerland)2014
Exploring PFGE for Detecting Large Plasmids in Campylobacter jejuni and Campylobacter coli Isolated from Various Retail Meats.
Article in Pathogens (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 28 citations in OpenAlex.
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- Plasmids of the incompatibility group FIBInternational microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Evaluation of core genome and whole genome multilocus sequence typing schemes forMicrobial genomics · 2023Article
- Comparison of Reference-Based Assembly and De Novo Assembly for Bacterial Plasmid Reconstruction and AMR Gene Localization inMicroorganisms · 2022Article
- The Distribution ofGenes · 2021Article
- Association between theAntibiotics (Basel, Switzerland) · 2021Article
- Natural Competence and Horizontal Gene Transfer in Campylobacter.Current topics in microbiology and immunology · 2021Article
- Draft Genome Sequence of Megaplasmid-Bearing Staphylococcus sciuri Strain B9-58B, Isolated from Retail Pork.Microbiology resource announcements · 2020Article
- Comparative Genomics of Plasmid-BearingFrontiers in microbiology · 2020Article
- Molecular Characterization ofFrontiers in microbiology · 2020Article
- The Whole-Genome Sequence of Plasmid-Bearing Staphylococcus argenteus Strain B3-25B from Retail Beef Liver Encodes the Type VII Secretion System and Several Virulence Factors.Microbiology resource announcements · 2019Article
- Draft Genome Sequences of Megaplasmid-Bearing Streptomyces sp. Strains BF-3 and 4F, Isolated from the Great Salt Plains of Oklahoma.Genome announcements · 2018Article
- Prevalence, Serotyping, Molecular Typing, and Antimicrobial Resistance ofFrontiers in microbiology · 2018Article
- Phylogenetic Relatedness Among Plasmids Harbored byFrontiers in microbiology · 2018Article
- Complete Genome Sequences of Plasmid-BearingGenome announcements · 2017Article
- The Whole-Genome Sequence ofGenome announcements · 2017Article
- Complete Genome Sequences of Plasmid-Bearing Multidrug-ResistantGenome announcements · 2017Article
- Complete Genome Sequences ofGenome announcements · 2017Article
- Complete Genome Sequences of Campylobacter jejuni Strains OD267 and WP2202 Isolated from Retail Chicken Livers and Gizzards Reveal the Presence of Novel 116-Kilobase and 119-Kilobase Megaplasmids with Type VI Secretion Systems.Genome announcements · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Campylobacter spp. is one of the most prevalent bacterial pathogens in retail meat, particularly poultry, and is a leading cause of diarrhea in humans. Studies related to Campylobacter large plasmids are limited in the literature possibly due to difficulty in isolating them using available alkaline lysis methods. The objectives of this study were to determine the prevalence of plasmids, particularly large ones, in Campylobacter spp. isolated from various Oklahoma retail meats, and to explore PFGE (Pulsed Field Gel Electrophoresis) as a tool in facilitating the detection of these plasmids. One hundred and eighty nine strains (94 Campylobacter jejuni and 95 Campylobacter coli) were screened for the presence of plasmids using both alkaline lysis and PFGE. Plasmids were detected in 119/189 (63%) using both methods. Most of the plasmids detected by alkaline lysis were smaller than 90 kb and only three were larger than 90 kb. Plasmids over 70 kb in size were detected in 33 more strains by PFGE of which 11 strains contained larger than 90 kb plasmids. Plasmids were more prevalent in Campylobacter coli (73.5%) than in Campylobacter jejuni (52%). BglII restriction analysis of plasmids isolated from 102 isolates revealed 42 different restriction patterns. In conclusion, PFGE was able to detect large plasmids up to 180 Kb in Campylobacter spp. which might have been missed if the alkaline lysis method was solely used. Campylobacter spp. isolated from retail meats harbor a diverse population of plasmids with variable sizes. To our knowledge, this is the first study to use PFGE to detect large plasmids in Campylobacter.
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Registered trials
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