ArticleBMC cell biology2014
In vivo imaging of Nematostella vectensis embryogenesis and late development using fluorescent probes.
Article in BMC cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Evaluation of a Novel Fluorescent Marker for Continuous Live Imaging of Preimplantation Embryos.Journal of developmental biology · 2026Article
- c-JUN controls microbial colonization via selective phagocytosis in the sea anemone Nematostella.Nature communications · 2026Article
- Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids.Biology open · 2025Article
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- Nuclear F-actin and Lamin A antagonistically modulate nuclear shape.Journal of cell science · 2022Article
- A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium.Nature communications · 2021Article
- Cytoskeletal and synaptic polarity of LWamide-like+ ganglion neurons in the sea anemoneThe Journal of experimental biology · 2020Article
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- Article
- Genomic analysis of the tryptome reveals molecular mechanisms of gland cell evolution.EvoDevo · 2019Article
- Microinjection to deliver protein, mRNA, and DNA into zygotes of the cnidarian endosymbiosis model Aiptasia sp.Scientific reports · 2018Article
- Germ layer-specific regulation of cell polarity and adhesion gives insight into the evolution of mesoderm.eLife · 2018Article
- Article
- Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis.Nature protocols · 2017Article
- Apical and basal epitheliomuscular F-actin dynamics duringBiology open · 2017Article
- Inducing Complete Polyp Regeneration from the Aboral Physa of the Starlet Sea Anemone Nematostella vectensis.Journal of visualized experiments : JoVE · 2017Article
- Do novel genes drive morphological novelty? An investigation of the nematosomes in the sea anemone Nematostella vectensis.BMC evolutionary biology · 2016Article
- Article
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7 authors.
Funding
Abstract
backgroundCnidarians are the closest living relatives to bilaterians and have been instrumental to studying the evolution of bilaterian properties. The cnidarian model, Nematostella vectensis, is a unique system in which embryology and regeneration are both studied, making it an ideal candidate to develop in vivo imaging techniques. Live imaging is the most direct way for quantitative and qualitative assessment of biological phenomena. Actin and tubulin are cytoskeletal proteins universally important for regulating many embryological processes but so far studies in Nematostella primarily focused on the localization of these proteins in fixed embryos.
resultsWe used fluorescent probes expressed in vivo to investigate the dynamics of Nematostella development. Lifeact-mTurquoise2, a fluorescent cyan F-actin probe, can be visualized within microvilli along the cellular surface throughout embryonic development and is stable for two months after injection. Co-expression of Lifeact-mTurquoise2 with End-Binding protein1 (EB1) fused to mVenus or tdTomato-NLS allows for the visualization of cell-cycle properties in real time. Utilizing fluorescent probes in vivo helped to identify a concentrated 'flash' of Lifeact-mTurquoise2 around the nucleus, immediately prior to cytokinesis in developing embryos. Moreover, Lifeact-mTurquoise2 expression in adult animals allowed the identification of various cell types as well as cellular boundaries.
conclusionThe methods developed in this manuscript provide an alternative protocol to investigate Nematostella development through in vivo cellular analysis. This study is the first to utilize the highly photo-stable florescent protein mTurquoise2 as a marker for live imaging. Finally, we present a clear methodology for the visualization of minute temporal events during cnidarian development.
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