ArticleNeuron2014
Vesicular glutamate transporters use flexible anion and cation binding sites for efficient accumulation of neurotransmitter.
Article in Neuron, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Substrate recognition and allosteric regulation of synaptic vesicle glutamate transporter VGLUT2.Nature structural & molecular biology · 2025Article
- Synaptophysin accelerates synaptic vesicle fusion by expanding the membrane upon neurotransmitter loading.Science advances · 2025Article
- Robust inhibitory glycinergic transmission and the effect of bafilomycin, folimycin and EIPA: lessons from the auditory brainstem.Frontiers in cellular neuroscience · 2025Article
- Article
- Vesicular glutamate transporters are HNature communications · 2023Article
- Neurotransmitter uptake of synaptic vesicles studied by X-ray diffraction.European biophysics journal : EBJ · 2022Article
- Diversity of function and mechanism in a family of organic anion transporters.Current opinion in structural biology · 2022Review
- A New Player in the Hippocampus: A Review on VGLUT3+ Neurons and Their Role in the Regulation of Hippocampal Activity and Behaviour.International journal of molecular sciences · 2022Review
- Roles of Endomembrane Alkali Cation/Proton Exchangers in Synaptic Function and Neurodevelopmental Disorders.Frontiers in physiology · 2022Review
- Isolation of Synaptic Vesicles from Mammalian Brain.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Opponent vesicular transporters regulate the strength of glutamatergic neurotransmission in a C. elegans sensory circuit.Nature communications · 2021Article
- Allosteric Inhibition of a Vesicular Glutamate Transporter by an Isoform-Specific Antibody.Biochemistry · 2021Article
- Inhibition of the Vesicular Glutamate Transporter (VGLUT) with Congo Red Analogs: New Binding Insights.Neurochemical research · 2021Article
- Physiological Perspectives on Molecular Mechanisms and Regulation of Vesicular Glutamate Transport: Lessons From Calyx of Held Synapses.Frontiers in cellular neuroscience · 2021Review
- Neurodegeneration Upon Dysfunction of Endosomal/Lysosomal CLC Chloride Transporters.Frontiers in cell and developmental biology · 2021Review
- The mechanism and regulation of vesicular glutamate transport: Coordination with the synaptic vesicle cycle.Biochimica et biophysica acta. Biomembranes · 2020Review
- Molecular, Structural, Functional, and Pharmacological Sites for Vesicular Glutamate Transporter Regulation.Molecular neurobiology · 2020Review
- Spike Activity Regulates Vesicle Filling at a Glutamatergic Synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Ion transport and regulation in a synaptic vesicle glutamate transporter.Science (New York, N.Y.) · 2020Article
- Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies.Scientific reports · 2020Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vesicular glutamate transporters (VGLUTs) accumulate the neurotransmitter glutamate in synaptic vesicles. Transport depends on a V-ATPase-dependent electrochemical proton gradient (ΔμH+) and requires chloride ions, but how chloride acts and how ionic and charge balance is maintained during transport is controversial. Using a reconstitution approach, we used an exogenous proton pump to drive VGLUT-mediated transport either in liposomes containing purified VGLUT1 or in synaptic vesicles fused with proton-pump-containing liposomes. Our data show that chloride stimulation can be induced at both sides of the membrane. Moreover, chloride competes with glutamate at high concentrations. In addition, VGLUT1 possesses a cation binding site capable of binding H+ or K+ ions, allowing for proton antiport or K+ / H+ exchange. We conclude that VGLUTs contain two anion binding sites and one cation binding site, allowing the transporter to adjust to the changing ionic conditions during vesicle filling without being dependent on other transporters or channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.