ArticleInternational journal of molecular sciences2014
Real-time qPCR identifies suitable reference genes for Borna disease virus-infected rat cortical neurons.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- A systematic review on the selection of reference genes for gene expression studies in rodents: are the classics the best choice?Molecular biology reports · 2024Pooled it
- Comparative Effects of Sertraline, Methotrexate, and Biologic Therapies on Circulating Inflammatory and Neurotrophic Mediators and Striatal Gene Expression in Collagen-Induced Arthritis.International journal of molecular sciences · 2026Article
- Evaluation of suitable reference genes for gene expression studies in the developing mouse cortex using RT-qPCR.BMC neuroscience · 2025Article
- Circular RNA expression profiles and functional predication after restraint stress in the amygdala of rats.Frontiers in molecular neuroscience · 2024Article
- Nucleoprotein (P40) Binding to 5HT2C Receptors (5HT2CR) is the Key Point in the Pathogenesis of BoDV-1-Infected Hosts.Neurochemical research · 2021Article
- Hydrogels for 3D Neural Tissue Models: Understanding Cell-Material Interactions at a Molecular Level.Frontiers in bioengineering and biotechnology · 2020Article
- Selection of Reliable Reference Genes for Analysis of Gene Expression in Spinal Cord during Rat Postnatal Development and after Injury.Brain sciences · 2019Article
- Genome-wide profiling of long noncoding RNA expression patterns and CeRNA analysis in mouse cortical neurons infected with different strains of borna disease virus.Genes & diseases · 2019Article
- TUBA1A mutations identified in lissencephaly patients dominantly disrupt neuronal migration and impair dynein activity.Human molecular genetics · 2019Article
- Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats.Neuropsychiatric disease and treatment · 2018Article
- Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells.PloS one · 2018Article
- Hyaluronan synthesis by developing cortical neurons in vitro.Scientific reports · 2017Article
- Identification of Reference Genes for Quantitative Gene Expression Studies in a Non-Model Tree Pistachio (Pistacia vera L.).PloS one · 2016Article
- GC-MS-Based Metabonomic Profiling Displayed Differing Effects of Borna Disease Virus Natural Strain Hu-H1 and Laboratory Strain V Infection in Rat Cortical Neurons.International journal of molecular sciences · 2015Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most commonly-used technique to identify gene expression profiles. The selection of stably expressed reference genes is a prerequisite to properly evaluating gene expression. Here, the suitability of commonly-used reference genes in normalizing RT-qPCR assays of mRNA expression in cultured rat cortical neurons infected with Borna disease virus (BDV) was assessed. The expressions of eight commonly-used reference genes were comparatively analyzed in BDV-infected rat cortical neurons and non-infected control neurons mainly across 9 and 12 days post-infection. These reference genes were validated by RT-qPCR and separately ranked by four statistical algorithms: geNorm, NormFinder, BestKeeper and the comparative delta-Ct method. Then, the RankAggreg package was used to construct consensus rankings. ARBP was found to be the most stable internal control gene at Day 9, and ACTB at Day 12. As the assessment of the validity of the selected reference genes confirms the suitability of applying a combination of the two most stable references genes, combining the two most stable genes for normalization of RT-qPCR studies in BDV-infected rat cortical neurons is recommended at each time point. This study can contribute to improving BDV research by providing the means by which to obtain more reliable and accurate gene expression measurements.
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