ArticleFrontiers in microbiology2014
A bioassay for the detection of benzimidazoles reveals their presence in a range of environmental samples.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- The Corrinoid Model for Dissecting Microbial Community Interactions Across Scales.Annual review of microbiology · 2025Review
- Naturally occurring variation in a cytochrome P450 modifies thiabendazole responses independently of beta-tubulin.PLoS pathogens · 2025Article
- Ligand cross-feeding resolves bacterial vitamin BNature · 2024Article
- Availability of vitamin BThe ISME journal · 2022Article
- Crystal structure, Hirshfeld and electronic transition analysis of 2-[(1Acta crystallographica. Section E, Crystallographic communications · 2021Article
- Sharing vitamins: Cobamides unveil microbial interactions.Science (New York, N.Y.) · 2020Review
- Flexible Cobamide Metabolism inJournal of bacteriology · 2020Article
- Metagenomic and chemical characterization of soil cobalamin production.The ISME journal · 2020Article
- Guided cobamide biosynthesis for heterologous production of reductive dehalogenases.Microbial biotechnology · 2019Article
- Uneven distribution of cobamide biosynthesis and dependence in bacteria predicted by comparative genomics.The ISME journal · 2019Article
- Extreme allelic heterogeneity at a Caenorhabditis elegans beta-tubulin locus explains natural resistance to benzimidazoles.PLoS pathogens · 2018Article
- Facile isolation of α-ribazole from vitamin BJournal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2018Article
- Metagenomics-guided analysis of microbial chemolithoautotrophic phosphite oxidation yields evidence of a seventh natural COProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Salmonella enterica synthesizes 5,6-dimethylbenzimidazolyl-(DMB)-α-riboside. Why some Firmicutes do not require the canonical DMB activation system to synthesize adenosylcobalamin.Molecular microbiology · 2017Article
- Decoding molecular interactions in microbial communities.FEMS microbiology reviews · 2016Review
- Cyanobacteria and Eukaryotic Algae Use Different Chemical Variants of Vitamin B12.Current biology : CB · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cobamides are a family of enzyme cofactors that include vitamin B12 (cobalamin) and are produced solely by prokaryotes. Structural variability in the lower axial ligand has been observed in cobamides produced by diverse organisms. Of the three classes of lower ligands, the benzimidazoles are uniquely found in cobamides, whereas the purine and phenolic bases have additional biological functions. Many organisms acquire cobamides by salvaging and remodeling cobamides or their precursors from the environment. These processes require free benzimidazoles for incorporation as lower ligands, though the presence of benzimidazoles in the environment has not been previously investigated. Here, we report a new purification method and bioassay to measure the total free benzimidazole content of samples from microbial communities and laboratory media components. The bioassay relies on the "calcofluor-bright" phenotype of a bluB mutant of the model cobalamin-producing bacterium Sinorhizobium meliloti. The concentrations of individual benzimidazoles in these samples were measured by liquid chromatography-tandem mass spectrometry. Several benzimidazoles were detected in subpicomolar to subnanomolar concentrations in host-associated and environmental samples. In addition, benzimidazoles were found to be common contaminants of laboratory media components. These results suggest that benzimidazoles present in the environment and in laboratory media have the potential to influence microbial metabolic activities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.