ArticleJournal of biosciences2014
Phosphorylation of DegU is essential for activation of amyE expression in Bacillus subtilis.
Article in Journal of biosciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- High-Level Expression of Sucrose Isomerase in Bacillus subtilis Through Expression Element Optimization and Fermentation Optimization.Applied biochemistry and biotechnology · 2025Article
- Automation-aided construction and characterization ofFrontiers in bioengineering and biotechnology · 2024Article
- Regulating Pathways ofInternational journal of molecular sciences · 2023Article
- DegU-mediated suppression of carbohydrate uptake inApplied and environmental microbiology · 2023Article
- The impact of PrsA over-expression on theFrontiers in microbiology · 2022Article
- An overview and future prospects of recombinant protein production in Bacillus subtilis.Applied microbiology and biotechnology · 2021Review
- The DegU Orphan Response Regulator Contributes to Heat Stress Resistance inFrontiers in cellular and infection microbiology · 2021Article
- High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system.Microbial cell factories · 2017Article
- Multigene disruption in undomesticated Bacillus subtilis ATCC 6051a using the CRISPR/Cas9 system.Scientific reports · 2016Article
- A novel expression vector for the secretion of abaecin in Bacillus subtilis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alpha-amylase (amyE) is one of the major exo-enzymes secreted by Bacillus subtilis during the post-exponential phase. The DegS-DegU two-component system regulates expression of majority of post-exponentially expressed genes in B. subtilis. It has been demonstrated that varying levels of the phosphorylated form of DegU (DegU-P) control different cellular processes. Exo-protease production is observed when effective concentration of DegU-P rises in the cell, whereas swarming motility is favoured at very low amounts of DegU-P. In this study we show that like other exo-proteases, expression of amyE is positively regulated by increase in DegU-P levels in the cell. We also demonstrate that residues at the DNA-binding helix-turn-helix (HTH) motif of DegU are necessary for the amyE expression. This observation is further reinforced by demonstrating the direct interaction of DegU on amyE promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.