ArticleMolecules (Basel, Switzerland)2014
Enzymatic cellulose hydrolysis: enzyme reusability and visualization of β-glucosidase immobilized in calcium alginate.
Article in Molecules (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 59 citations in OpenAlex.
- The Isolation, Identification and Immobilization Method of Three Novel Enzymes with Diosgenin-Producing Activity Derived from anInternational journal of molecular sciences · 2023Article
- Bioethanol Production from Lignocellulosic Biomass-Challenges and Solutions.Molecules (Basel, Switzerland) · 2022Review
- Immobilization ofInternational journal of molecular sciences · 2022Article
- Optimization of Cellulase Immobilization with Sodium Alginate-Polyethylene for Enhancement of Enzymatic Hydrolysis of Microcrystalline Cellulose Using Response Surface Methodology.Applied biochemistry and biotechnology · 2021Article
- Biodegradation of Crystalline Cellulose Nanofibers by Means of Enzyme Immobilized-Alginate Beads and Microparticles.Polymers · 2020Article
- Enhanced Catalytic Performance of Trichoderma reesei Cellulase Immobilized on Magnetic Hierarchical Porous Carbon Nanoparticles.The protein journal · 2019Article
- Biochemical Characteristics of Microbial Enzymes and Their Significance from Industrial Perspectives.Molecular biotechnology · 2019Review
- Transforming food waste: how immobilized enzymes can valorize waste streams into revenue streams.NPJ science of food · 2018Review
- Special Issue: Enzyme Immobilization 2016.Molecules (Basel, Switzerland) · 2017Article
- Prevention of Bacterial Contamination of a Silica Matrix Containing Entrapped β-Galactosidase through the Action of Covalently Bound Lysozymes.Molecules (Basel, Switzerland) · 2017Article
- Novel Magnetic Cross-Linked Cellulase Aggregates with a Potential Application in Lignocellulosic Biomass Bioconversion.Molecules (Basel, Switzerland) · 2017Article
- Immobilization of Glycoside Hydrolase Families GH1, GH13, and GH70: State of the Art and Perspectives.Molecules (Basel, Switzerland) · 2016Review
- Molecular Structural Basis for the Cold Adaptedness of the Psychrophilic β-Glucosidase BglU in Micrococcus antarcticus.Applied and environmental microbiology · 2016Article
- Editorial: Special issue--Enzyme immobilization.Molecules (Basel, Switzerland) · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The high cellulase enzyme dosages required for hydrolysis of cellulose is a major cost challenge in lignocellulosic ethanol production. One method to decrease the enzyme dosage and increase biocatalytic productivity is to re-use β-glucosidase (BG) via immobilization. In the present research, glutaraldehyde cross-linked BG was entrapped in calcium alginate gel particles. More than 60% of the enzyme activity could be recovered under optimized conditions, and glutaraldehyde cross-linking decreased leakage of BG from the calcium alginate particles. The immobilized BG aggregates were visualized by confocal laser scanning microscopy (CLSM). The CLSM images, which we believe are the first to be published, corroborate that more BG aggregates were entrapped in the matrix when the enzymes were cross-linked by glutaraldehyde as opposed to when they are not cross-linked. The particles with the immobilized BG were recycled for cellulase catalyzed hydrolysis of Avicel. No significant loss in BG activity was observed for up to 20 rounds of reaction recycle steps of the BG particles of 48 h each, verifying a significant stabilization of the BG by immobilization. Similar high glucose yields were obtained by one round of enzymatic hydrolysis of hydrothermally pretreated barley straw during a 72 h reaction with immobilized BG and free BG.
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