ArticleFrontiers in cellular and infection microbiology2014
Comparison of bacterial culture and 16S rRNA community profiling by clonal analysis and pyrosequencing for the characterization of the dentine caries-associated microbiome.
Article in Frontiers in cellular and infection microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 65 citations in OpenAlex.
- Long-term remineralizing effect of MI Paste Plus on regression of early caries after orthodontic fixed appliance treatment: a 12-month follow-up randomized controlled trial.European journal of orthodontics · 2018Trial
- Bacterial community associated with the surface and inside of centipede forcipules: Identification and characterization.PloS one · 2026Article
- Differential microbiome response to sucrose in caries-free and caries-affected individuals.AMB Express · 2025Article
- Altered oral health and microbiota in drug-free patients with schizophrenia.BMC psychiatry · 2025Observational
- Structural and functional changes in the oral microbiome of patients with craniofacial microsomia.Scientific reports · 2025Article
- The forgotten link: how the oral microbiome shapes childhood growth and development.Frontiers in oral health · 2025Review
- Dental caries shallow-layer microbe harvest and data display method shows taxa pre-harvest spatial positions, absolute and relative abundance and diversity related to lesion pulpal depth.Journal of oral microbiology · 2025Article
- Characterising the Tasmanian devil (Sarcophilus harrisii) pouch microbiome in lactating and non-lactating females.Scientific reports · 2024Article
- Bibliometric analysis of the association between periodontal disease and cardiovascular disease.Heliyon · 2024Article
- The Evolving Microbiome of Dental Caries.Microorganisms · 2024Review
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- From Mouth to Brain: Distinct Supragingival Plaque Microbiota Composition in Cerebral Palsy Children With Caries.Frontiers in cellular and infection microbiology · 2022Article
- Sodium Butyrate Attenuates Taurocholate-Induced Acute Pancreatitis by Maintaining Colonic Barrier and Regulating Gut Microorganisms in Mice.Frontiers in physiology · 2022Article
- Old age and other factors associated with salivary microbiome variation.BMC oral health · 2021Article
- Role of microbes in the pathogenesis of neuropsychiatric disorders.Frontiers in neuroendocrinology · 2021Review
- 919 Syrup Alleviates Postpartum Depression by Modulating the Structure and Metabolism of Gut Microbes and Affecting the Function of the Hippocampal GABA/Glutamate System.Frontiers in cellular and infection microbiology · 2021Article
- Oral Dysbiosis and Inflammation in Parkinson's Disease.Journal of Parkinson's disease · 2021Article
- Microbes involved in arsenic mobilization and respiration: a review on isolation, identification, isolates and implications.Environmental geochemistry and health · 2020Review
- Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study.International journal of environmental research and public health · 2020Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culture-independent analyses have greatly expanded knowledge regarding the composition of complex bacterial communities including those associated with oral diseases. A consistent finding from such studies, however, has been the under-reporting of members of the phylum Actinobacteria. In this study, five pairs of broad range primers targeting 16S rRNA genes were used in clonal analysis of 6 samples collected from tooth lesions involving dentine in subjects with active caries. Samples were also subjected to cultural analysis and pyrosequencing by means of the 454 platform. A diverse bacterial community of 229 species-level taxa was revealed by culture and clonal analysis, dominated by representatives of the genera Prevotella, Lactobacillus, Selenomonas, and Streptococcus. The five most abundant species were: Lactobacillus gasseri, Prevotella denticola, Alloprevotella tannerae, S. mutans and Streptococcus sp. HOT 070, which together made up 31.6 % of the sequences. Two samples were dominated by lactobacilli, while the remaining samples had low numbers of lactobacilli but significantly higher numbers of Prevotella species. The different primer pairs produced broadly similar data but proportions of the phylum Bacteroidetes were significantly higher when primer 1387R was used. All of the primer sets underestimated the proportion of Actinobacteria compared to culture. Pyrosequencing analysis of the samples was performed to a depth of sequencing of 4293 sequences per sample which were identified to 264 species-level taxa, and resulted in significantly higher coverage estimates than the clonal analysis. Pyrosequencing, however, also underestimated the relative abundance of Actinobacteria compared to culture.
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