ArticleInternational journal of nanomedicine2014
Incorporation of osteogenic and angiogenic small interfering RNAs into chitosan sponge for bone tissue engineering.
Article in International journal of nanomedicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Versatility of Chitosan-Based Biomaterials and Their Use as Scaffolds for Tissue Regeneration.TheScientificWorldJournal · 2017Pooled it
- Programmable biomaterials for bone regeneration.Materials today. Bio · 2024Review
- Chitosan-Based Scaffolds for Facilitated Endogenous Bone Re-Generation.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Non-coding RNA delivery for bone tissue engineering: Progress, challenges, and potential solutions.iScience · 2022Review
- Translational Application of 3D Bioprinting for Cartilage Tissue Engineering.Bioengineering (Basel, Switzerland) · 2021Review
- Natural Polymers for the Maintenance of Oral Health: Review of Recent Advances and Perspectives.International journal of molecular sciences · 2021Review
- The construction of a novel xenograft bovine bone scaffold, (DSS)6-liposome/CKIP-1 siRNA/calcine bone and its osteogenesis evaluation on skull defect in rats.Journal of orthopaedic translation · 2021Article
- Designing biomaterials for the delivery of RNA therapeutics to stimulate bone healing.Materials today. Bio · 2021Review
- Biophysical and Biochemical Cues of Biomaterials Guide Mesenchymal Stem Cell Behaviors.Frontiers in cell and developmental biology · 2021Review
- An Overview of RNA-Based Scaffolds for Osteogenesis.Frontiers in molecular biosciences · 2021Review
- Challenges in Bone Tissue Regeneration: Stem Cell Therapy, Biofunctionality and Antimicrobial Properties of Novel Materials and Its Evolution.International journal of molecular sciences · 2020Review
- RNA-based scaffolds for bone regeneration: application and mechanisms of mRNA, miRNA and siRNA.Theranostics · 2020Review
- Small non-coding RNAs-based bone regulation and targeting therapeutic strategies.Molecular and cellular endocrinology · 2017Review
- Gene therapy for bone tissue engineering.Tissue engineering and regenerative medicine · 2016Article
- Gene therapy for bone engineering.Frontiers in bioengineering and biotechnology · 2015Review
- Chitosan-tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity.International journal of nanomedicine · 2015Article
- Strategies to Stimulate Mobilization and Homing of Endogenous Stem and Progenitor Cells for Bone Tissue Repair.Frontiers in bioengineering and biotechnology · 2015Review
- 3D Bioprinting tissue analogs: Current development and translational implications.Journal of tissue engineeringReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineered bone substitutes are being extensively explored in response to growing demand. However, the angiogenesis that occurs during bone formation is often overlooked in scaffold design. In this novel study, we incorporated two small interfering RNAs (siRNAs), ie, small interfering RNA targets casein kinase 2 interaction protein 1 (siCkip-1) and small interfering RNA targets soluble VEGF receptor 1 (siFlt-1), which can promote osteogenesis and angiogenesis, into a chitosan sponge. This scaffold could maintain siRNAs for over 2 weeks in neutral phosphate-buffered saline and degraded rapidly in the presence of lysozyme. The chitosan sponge with siCkip-1 and siFlt-1 in vitro bioactivity was investigated using mesenchymal stem cells. Target genes were significantly suppressed, and osteocalcin, alkaline phosphatase, and vascular endothelial growth factor were significantly upregulated. Alizarin Red staining revealed that mineralization of the extracellular matrix was markedly enhanced by dual transfection. Further analysis by immunofluorescence confirmed that the siRNA-modified scaffold simultaneously improved the expression of osteocalcin and von Willebrand factor. In vivo testing in a skull critical-size defect model showed marked bone regeneration in rats treated with siCkip-1 and siFlt-1. In conclusion, chitosan sponge containing osteogenic and angiogenic siRNAs may be used as a scaffold for bone regeneration. The dual siRNA concept may also be useful in the biofunctionalization of other materials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.