ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
Proton-mediated block of Ca2+ channels during multivesicular release regulates short-term plasticity at an auditory hair cell synapse.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Electroreceptors with a different kind of buzz.The Journal of physiology · 2026Article
- Role of the C-terminal domain in modifying pH-dependent regulation of CaChannels (Austin, Tex.) · 2025Article
- Proton-triggered rearrangement of the AMPA receptor N-terminal domains impacts receptor kinetics and synaptic localization.Nature structural & molecular biology · 2024Article
- Postsynaptic Calcium Extrusion at the Mouse Neuromuscular Junction Alkalinizes the Synaptic Cleft.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- T-Type CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Computational modeling predicts ephemeral acidic microdomains in the glutamatergic synaptic cleft.Biophysical journal · 2021Article
- Using ephaptic coupling to estimate the synaptic cleft resistivity of the calyx of Held synapse.PLoS computational biology · 2021Article
- Phase-Locking Requires Efficient CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- A new tool to sense pH changes at the neuromuscular junction synaptic cleft.Scientific reports · 2020Article
- Neuronal Glutamatergic Synaptic Clefts Alkalinize Rather Than Acidify during Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Synaptic cleft microenvironment influences potassium permeation and synaptic transmission in hair cells surrounded by calyx afferents in the turtle.The Journal of physiology · 2020Article
- Vesicular Glutamatergic Transmission in Noise-Induced Loss and Repair of Cochlear Ribbon Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Clustered CaCell reports · 2018Article
- Voltage-Gated Calcium Channels: Key Players in Sensory Coding in the Retina and the Inner Ear.Physiological reviews · 2018Review
- Protons as Messengers of Intercellular Communication in the Nervous System.Frontiers in cellular neuroscience · 2018Review
- Differential Phase Arrangement of Cellular Clocks along the Tonotopic Axis of the Mouse Cochlea Ex Vivo.Current biology : CB · 2017Article
- Distinct Actions of Voltage-Activated CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Different CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Acid-Sensing Ion Channels Activated by Evoked Released Protons Modulate Synaptic Transmission at the Mouse Calyx of Held Synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- The Coupling between CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Synaptic vesicles release both neurotransmitter and protons during exocytosis, which may result in a transient acidification of the synaptic cleft that can block Ca(2+) channels located close to the sites of exocytosis. Evidence for this effect has been reported for retinal ribbon-type synapses, but not for hair cell ribbon synapses. Here, we report evidence for proton release from bullfrog auditory hair cells when they are held at more physiological, in vivo-like holding potentials (Vh = -60 mV) that facilitate multivesicular release. During paired recordings of hair cells and afferent fibers, L-type voltage-gated Ca(2+) currents showed a transient block, which was highly correlated with the EPSC amplitude (or the amount of glutamate release). This effect was masked at Vh = -90 mV due to the presence of a T-type Ca(2+) current and blocked by strong pH buffering with HEPES or TABS. Increasing vesicular pH with internal methylamine in hair cells also abolished the transient block. High concentrations of intracellular Ca(2+) buffer (10 mm BAPTA) greatly reduced exocytosis and abolished the transient block of the Ca(2+) current. We estimate that this transient block is due to the rapid multivesicular release of ∼600-1300 H(+) ions per synaptic ribbon. Finally, during a train of depolarizing pulses, paired pulse plasticity was significantly changed by using 40 mm HEPES in addition to bicarbonate buffer. We propose that this transient block of Ca(2+) current leads to more efficient exocytosis per Ca(2+) ion influx and it may contribute to spike adaptation at the auditory nerve.
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