ArticlePlant physiology2015
Promotion of testa rupture during garden cress germination involves seed compartment-specific expression and activity of pectin methylesterases.
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Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41citing papers in PubMed
8.4field-weighted citation impact, top 3% of its field
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41 citing papers in PubMed, 74 citations in OpenAlex.
- The trade-off mechanism between fiber elongation and seed vigor formation mediated by the RCD1-MYC3 module in cotton.Science China. Life sciences · 2026Article
- Pectin dynamics of alfalfa pollen tube elongation in response to low boron based on proteomics.BMC plant biology · 2026Article
- Holistic overview of regulatory networks governing seed dormancy and germination in plants.Science China. Life sciences · 2026Review
- Distinct Molecular Biomechanical Mechanisms Inhibit Endosperm Cell-Wall Weakening and Seed Germination at Cold and Warm Nonoptimal Temperatures.Plant, cell & environment · 2025Article
- Analysis of xyloglucan metabolism mutants highlights the prominent role of xylose cleavage in seed dormancy.The Plant journal : for cell and molecular biology · 2025Article
- Chromosome doubling increases PECTIN METHYLESTERASE 2 expression, biomass, and osmotic stress tolerance in kiwifruit.Plant physiology · 2024Article
- Pectin Remodeling and Involvement of AtPME3 in the Parasitic Plant-Plant Interaction,Plants (Basel, Switzerland) · 2024Article
- The dimorphic diaspore model Aethionema arabicum (Brassicaceae): Distinct molecular and morphological control of responses to parental and germination temperatures.The Plant cell · 2024Article
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- Pectin methylesterase 31 is transcriptionally repressed by ABI5 to negatively regulate ABA-mediated inhibition of seed germination.Frontiers in plant science · 2024Article
- Effect of Reactive Oxygen Scavenger N,N'-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize.International journal of molecular sciences · 2023Article
- Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton.BMC plant biology · 2022Article
- Molecular mechanisms of seed dormancy release by gas plasma-activated water technology.Journal of experimental botany · 2022Article
- The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and InhibitsInternational journal of molecular sciences · 2022Article
- Cold-induced secondary dormancy and its regulatory mechanisms in Beta vulgaris.Plant, cell & environment · 2022Article
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- Cutin:cutin-acid endo-transacylase (CCT), a cuticle-remodelling enzyme activity in the plant epidermis.The Biochemical journal · 2021Article
- Dead but Not Dead End: Multifunctional Role of Dead Organs Enclosing Embryos in Seed Biology.International journal of molecular sciences · 2020Review
- Extreme drought alters progeny dispersal unit properties of winter wild oat (Avena sterilis L.).Planta · 2020Article
- The seed water content as a time-independent physiological trait during germination in wild tree species such as Ceiba aesculifolia.Scientific reports · 2020Article
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Authors and funding
17 authors at 9 institutions in 7 countries.
Claudia SchelerBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Karin WeitbrechtBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Simon P PearceBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Anthony HampsteadBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Annette Büttner-MainikBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Kieran J D LeeBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Antje VoegeleBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Krystyna OraczBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Bas J W DekkersBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Xiaofeng WangBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Andrew T A WoodBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Leónie BentsinkBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
John R KingBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
J Paul KnoxBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Michael J HoldsworthBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Kerstin MüllerBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.).
Gerhard Leubner-MetzgerBotany and Plant Physiology, Institute for Biology II, Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany (C.S., K.W., A.B.-M., K.O., G.L.-M.);Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, D-85764 Neuherberg, Germany (C.S.);Staatliches Weinbauinstitut Freiburg, D-79104 Freiburg, Germany (K.W.);Centre for Plant Integrative Biology (S.P.P., A.H., A.T.A.W., J.R.K., M.J.H.) and Division of Plant and Crop Science (S.P.P., M.J.H., K.M.), School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire LE12 5RD, United Kingdom;School of Mathematical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom (S.P.P., A.H., A.T.A.W., J.R.K.)Agroscope, Institute for Plant Production Sciences, Seed Quality, CH-8046 Zurich, Switzerland (A.B.-M.);Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (K.J.D.L., J.P.K.);National Institute for Health Research Trainees Coordinating Centre, Leeds Innovation Centre, Leeds LS2 9DF, United Kingdom (K.J.D.L.);School of Biological Sciences, Plant Molecular Science and Centre for Systems and Synthetic Biology, Royal Holloway, University of London, Egham, Surrey TW20 0EX, United Kingdom (A.V., G.L.-M.);Department of Plant Physiology, Warsaw University of Life Sciences, 02-776, Warsaw, Poland (K.O.);Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University and Research Centre, NL-6708 PB Wageningen, The Netherlands (B.J.W.D., L.B.);College of Life Sciences, South China Agricultural University, Guangzhou 510642, China (X.W.); andLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, CZ-783 71 Olomouc, Czech Republic (G.L.-M.) gerhard.leubner@rhul.ac.uk.
University of Nottingham · GBRoyal Holloway University of London · GBUniversity of Leeds · GBWageningen University & Research · NLAgroscope · CHHelmholtz Zentrum München · DESouth China Agricultural University · CNStaatliches Weinbauinstitut Freiburg · DEWarsaw University of Life Sciences · PL
Funding
Biotechnology and Biological Sciences Research Council BB/G02488X/1Biotechnology and Biological Sciences Research Council BB/G024898/1
Abstract
Pectin methylesterase (PME) controls the methylesterification status of pectins and thereby determines the biophysical properties of plant cell walls, which are important for tissue growth and weakening processes. We demonstrate here that tissue-specific and spatiotemporal alterations in cell wall pectin methylesterification occur during the germination of garden cress (Lepidium sativum). These cell wall changes are associated with characteristic expression patterns of PME genes and resultant enzyme activities in the key seed compartments CAP (micropylar endosperm) and RAD (radicle plus lower hypocotyl). Transcriptome and quantitative real-time reverse transcription-polymerase chain reaction analysis as well as PME enzyme activity measurements of separated seed compartments, including CAP and RAD, revealed distinct phases during germination. These were associated with hormonal and compartment-specific regulation of PME group 1, PME group 2, and PME inhibitor transcript expression and total PME activity. The regulatory patterns indicated a role for PME activity in testa rupture (TR). Consistent with a role for cell wall pectin methylesterification in TR, treatment of seeds with PME resulted in enhanced testa permeability and promoted TR. Mathematical modeling of transcript expression changes in germinating garden cress and Arabidopsis (Arabidopsis thaliana) seeds suggested that group 2 PMEs make a major contribution to the overall PME activity rather than acting as PME inhibitors. It is concluded that regulated changes in the degree of pectin methylesterification through CAP- and RAD-specific PME and PME inhibitor expression play a crucial role during Brassicaceae seed germination.
Indexed as
Carboxylic Ester HydrolasesEndospermGene Expression ProfilingGene Expression Regulation, PlantGerminationHypocotylLepidium sativumPlant ProteinsReal-Time Polymerase Chain ReactionSeedsCarboxylic Ester HydrolasespectinesterasePlant Proteins
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