ArticleMolecular biology of the cell2015
Phospholipase D1 facilitates second-phase myoblast fusion and skeletal muscle regeneration.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
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- Skeletal Muscle Regeneration in Cardiotoxin-Induced Muscle Injury Models.International journal of molecular sciences · 2022Review
- Self-transducible LRS-UNE-L peptide enhances muscle regeneration.Journal of cachexia, sarcopenia and muscle · 2022Article
- Muscle regeneration controlled by a designated DNA dioxygenase.Cell death & disease · 2021Article
- Simulated microgravity inhibits C2C12 myogenesis via phospholipase D2-induced Akt/FOXO1 regulation.Scientific reports · 2019Article
- Comparative Transcriptome and Methylome Analysis in Human Skeletal Muscle Anabolism, Hypertrophy and Epigenetic Memory.Scientific reports · 2019Article
- Transcriptomic analyses reveal rhythmic and CLOCK-driven pathways in human skeletal muscle.eLife · 2018Article
- A novel in vitro model for the assessment of postnatal myonuclear accretion.Skeletal muscle · 2018Article
- Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes.International journal of nanomedicine · 2016Article
- Article
- Physiological and pathophysiological roles for phospholipase D.Journal of lipid research · 2015Review
- HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth.Journal of molecular cell biology · 2015Article
- The phospholipase D superfamily as therapeutic targets.Trends in pharmacological sciences · 2015Review
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Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myoblast differentiation and fusion is a well-orchestrated multistep process that is essential for skeletal muscle development and regeneration. Phospholipase D1 (PLD1) has been implicated in the initiation of myoblast differentiation in vitro. However, whether PLD1 plays additional roles in myoblast fusion and exerts a function in myogenesis in vivo remains unknown. Here we show that PLD1 expression is up-regulated in myogenic cells during muscle regeneration after cardiotoxin injury and that genetic ablation of PLD1 results in delayed myofiber regeneration. Myoblasts derived from PLD1-null mice or treated with PLD1-specific inhibitor are unable to form mature myotubes, indicating defects in second-phase myoblast fusion. Concomitantly, the PLD1 product phosphatidic acid is transiently detected on the plasma membrane of differentiating myocytes, and its production is inhibited by PLD1 knockdown. Exogenous lysophosphatidylcholine, a key membrane lipid for fusion pore formation, partially rescues fusion defect resulting from PLD1 inhibition. Thus these studies demonstrate a role for PLD1 in myoblast fusion during myogenesis in which PLD1 facilitates the fusion of mononuclear myocytes with nascent myotubes.
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