Evidence map›Paper›PMID 25428257›Full record

ArticleEpidemiology and infection2015

Multilocus genotypic characterization of Escherichia coli O157:H7 recovered from food sources.

M M Elhadidy, W F Elkhatib

Abstract read
In one paragraph

Article in Epidemiology and infection, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

M M ElhadidyDepartment of Bacteriology,Mycology and Immunology,Faculty of Veterinary Medicine,Mansoura University,Mansoura,Egypt.
W F ElkhatibDepartment of Microbiology & Immunology,Faculty of Pharmacy,Ain Shams University,African Union Organization,St. Abbassia,Cairo,Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Escherichia coli O157:H7 strains (n = 33) recovered from different food sources in Egypt were characterized using molecular assays to identify strain genotypes associated with various levels of pathogenic potential. Genotypic characterization included: lineage-specific polymorphism assay (LSPA-6), Shiga-toxin-encoding bacteriophage insertion site assay (SBI), clade 8 typing, Tir (A255 T) polymorphism, and variant analysis of Shiga toxin 2 gene (Stx 2a and Stx 2c), and anti-terminator Q genes (Q 933 and Q 21). Genotypes LI/II (76%), SBI 1 (60·6%), clade 8 (69·7%), Tir (255 T) (72·7%) and Stx 2c (45·5%) were found to be significantly more frequent compared to other genetic markers in the strains analysed. Multivariable analysis revealed a significant association between LPSA-6 and clade types as well as Tir (A255 T). To the best of our knowledge, this is the first study to report the characterization of these genetic markers in E. coli O157:H7 strains in the Middle East and Africa.

Indexed as

Food MicrobiologyAnimalsCheeseDNA, BacterialEgyptEscherichia coli InfectionsEscherichia coli O157Escherichia coli ProteinsGenes, vifGenotypeHumansMeatMilkReceptors, Cell SurfaceShiga ToxinVirulence FactorsDNA, BacterialEscherichia coli ProteinsReceptors, Cell SurfaceShiga ToxinTir protein, E coliVirulence FactorsCharacterizationE. coli O157:H7foodgenotypes

Identifiers

PMID25428257
PMCPMC9150932

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.