ArticleClinical and vaccine immunology : CVI2015
Characterization of cross-reactive norovirus-specific monoclonal antibodies.
Article in Clinical and vaccine immunology : CVI, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Mutational analysis of human norovirus VP2 elucidates critical molecular interactions for virus assembly.Journal of virology · 2026Article
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target.ACS applied materials & interfaces · 2024Article
- Engineered Yeast Displaying Specific Norovirus-Binding Nanobodies for the Concentration and Detection of Human Norovirus in Food Matrix.Journal of agricultural and food chemistry · 2023Article
- Article
- Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus-Ligand Binding.Viruses · 2022Review
- Broadly cross-reactive human antibodies that inhibit genogroup I and II noroviruses.Nature communications · 2021Article
- Histo-blood group antigens of glycosphingolipids predict susceptibility of human intestinal enteroids to norovirus infection.The Journal of biological chemistry · 2020Article
- A Survey of Analytical Techniques for Noroviruses.Foods (Basel, Switzerland) · 2020Review
- Review
- Use of rabbit antibodies raised againstThe Indian journal of medical research · 2017Article
- Deep sequencing of phage-displayed peptide libraries reveals sequence motif that detects norovirus.Protein engineering, design & selection : PEDS · 2017Article
- Identification and Characterization of Single-Chain Antibodies that Specifically Bind GI Noroviruses.PloS one · 2017Article
- Replication of Human Norovirus RNA in Mammalian Cells Reveals Lack of Interferon Response.Journal of virology · 2016Article
- Epidemiology of Norovirus Infection Among Immunocompromised Patients at a Tertiary Care Research Hospital, 2010-2013.Open forum infectious diseases · 2016Article
- Frequent Use of the IgA Isotype in Human B Cells Encoding Potent Norovirus-Specific Monoclonal Antibodies That Block HBGA Binding.PLoS pathogens · 2016Article
- Norovirus Antigen Detection with a Combination of Monoclonal and Single-Chain Antibodies.Journal of clinical microbiology · 2015Article
- Mapping broadly reactive norovirus genogroup I and II monoclonal antibodies.Clinical and vaccine immunology : CVI · 2015Article
- Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Noroviruses (NoVs) commonly cause acute gastroenteritis outbreaks. Broadly reactive diagnostic assays are essential for rapid detection of NoV infections. We previously generated a panel of broadly reactive monoclonal antibodies (MAbs). We characterized MAb reactivities by use of virus-like particles (VLPs) from 16 different NoV genotypes (6 from genogroup I [GI], 9 from GII, and 1 from GIV) coating a microtiter plate (direct enzyme-linked immunosorbent assay [ELISA]) and by Western blotting. MAbs were genotype specific or recognized multiple genotypes within a genogroup and between genogroups. We next applied surface plasmon resonance (SPR) analysis to measure MAb dissociation constants (Kd) as a surrogate for binding affinity; a Kd level of <10 nM was regarded as indicating strong binding. Some MAbs did not interact with the VLPs by SPR analysis. To further assess this lack of MAb-VLP interaction, the MAbs were evaluated for the ability to identify NoV VLPs in a capture ELISA. Those MAbs for which a Kd could not be measured by SPR analysis also failed to capture the NoV VLPs; in contrast, those with a measurable Kd gave a positive signal in the capture ELISA. Thus, some broadly cross-reactive epitopes in the VP1 protruding domain may be partially masked on intact particles. One MAb, NV23, was able to detect genogroup I, II, and IV VLPs from 16 genotypes tested by sandwich ELISA, and it successfully detected NoVs in stool samples positive by real-time reverse transcription-PCR when the threshold cycle (CT) value was <31. Biochemical analyses of MAb reactivity, including SPR analysis, identified NV23 as a broadly reactive ligand for application in norovirus diagnostic assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.