ArticleBMC developmental biology2014
A vertebrate-conserved cis-regulatory module for targeted expression in the main hypothalamic regulatory region for the stress response.
Article in BMC developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Article
- Altered glucocorticoid reactivity and behavioral phenotype in rx3-/- larval zebrafish.Frontiers in endocrinology · 2023Article
- The homeodomain transcription factor Orthopedia is involved in development of the Drosophila hindgut.Hereditas · 2020Article
- Stress-mediated convergence of splicing landscapes in male and female rock doves.BMC genomics · 2020Article
- Zebrafish oxytocin neurons drive nocifensive behavior via brainstem premotor targets.Nature neuroscience · 2019Article
- Performance on innate behaviour during early development as a function of stress level.Scientific reports · 2017Article
- iDamIDseq and iDEAR: an improved method and computational pipeline to profile chromatin-binding proteins.Development (Cambridge, England) · 2016Article
- Optogenetically enhanced pituitary corticotroph cell activity post-stress onset causes rapid organizing effects on behaviour.Nature communications · 2016Article
- Motivated state control in larval zebrafish: behavioral paradigms and anatomical substrates.Journal of neurogenetics · 2016Review
- The Severity of Acute Stress Is Represented by Increased Synchronous Activity and Recruitment of Hypothalamic CRH Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Role of developmental factors in hypothalamic function.Frontiers in neuroanatomy · 2015Review
- Article
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5 authors.
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Abstract
backgroundThe homeodomain transcription factor orthopedia (Otp) is an evolutionarily conserved regulator of neuronal fates. In vertebrates, Otp is necessary for the proper development of different regions of the brain and is required in the diencephalon to specify several hypothalamic cell types, including the cells that control the stress response. To understand how this widely expressed transcription factor accomplishes hypothalamus-specific functions, we performed a comprehensive screening of otp cis-regulatory regions in zebrafish.
resultsHere, we report the identification of an evolutionarily conserved vertebrate enhancer module with activity in a restricted area of the forebrain, which includes the region of the hypothalamus that controls the stress response. This region includes neurosecretory cells producing Corticotropin-releasing hormone (Crh), Oxytocin (Oxt) and Arginine vasopressin (Avp), which are key components of the stress axis. Lastly, expression of the bacterial nitroreductase gene under this specific enhancer allowed pharmacological attenuation of the stress response in zebrafish larvae.
conclusionVertebrates share many cellular and molecular components of the stress response and our work identified a striking conservation at the cis-regulatory level of a key hypothalamic developmental gene. In addition, this enhancer provides a useful tool to manipulate and visualize stress-regulatory hypothalamic cells in vivo with the long-term goal of understanding the ontogeny of the stress axis in vertebrates.
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