ArticleMolecular and cellular biology1989
Differentiation-responsive elements in the 5' region of the mouse tissue plasminogen activator gene confer two-stage regulation by retinoic acid and cyclic AMP in teratocarcinoma cells.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 90 citations in OpenAlex.
- Retinoids regulate human amniotic tissue-type plasminogen activator gene by a two-step mechanism.Journal of cellular and molecular medicine · 2010Article
- GATA factors and androgen receptor collaborate to transcriptionally activate the Rhox5 homeobox gene in Sertoli cells.Molecular and cellular biology · 2008Article
- Absence of tissue plasminogen activator gene or activity impairs mouse cerebellar motor learning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Synergistic transcriptional activation of the mouse urokinase plasminogen activator (uPA) gene and of its enhancer activator protein 1 (AP1) site by cAMP and retinoic acid.The Biochemical journal · 1998Article
- Biochemical, genetic, and metabolic adaptations of tumor cells that express the typical multidrug-resistance phenotype. Reversion by new therapies.Journal of bioenergetics and biomembranes · 1997Review
- Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts.Molecular and cellular biology · 1997Article
- Cell-type specific DNA-protein interactions at the tissue-type plasminogen activator promoter in human endothelial and HeLa cells in vivo and in vitro.Nucleic acids research · 1997Article
- The kinetics of induction of Hox1.6 and C-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells.In vitro cellular & developmental biology. Animal · 1995Article
- AP-1, ETS, and transcriptional silencers regulate retinoic acid-dependent induction of keratin 18 in embryonic cells.Molecular and cellular biology · 1994Article
- Different binding specificities and transactivation of variant CRE's by CREB complexes.Nucleic acids research · 1994Article
- Article
- Expression of a 91-kilodalton PEA3-binding protein is down-regulated during differentiation of F9 embryonal carcinoma cells.Molecular and cellular biology · 1992Article
- Thrombomodulin gene regulation by cAMP and retinoic acid in F9 embryonal carcinoma cells.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Cyclic AMP response element-binding protein and the catalytic subunit of protein kinase A are present in F9 embryonal carcinoma cells but are unable to activate the somatostatin promoter.Molecular and cellular biology · 1992Article
- Regulation of E2F/cyclin A-containing complex upon retinoic acid-induced differentiation of teratocarcinoma cells.Gene expression · 1992Article
- PEA1 and PEA3 enhancer elements are primary components of the polyomavirus late transcription initiator element.Journal of virology · 1991Article
- In vitro analysis of the tissue plasminogen activator promoter reveals a GC box-binding activity present in murine brain but undetectable in kidney and liver.Molecular and cellular biology · 1991Article
- E1A dependent up-regulation of c-jun/AP-1 activity.Nucleic acids research · 1991Article
- The cellular transcription factor CREB corresponds to activating transcription factor 47 (ATF-47) and forms complexes with a group of polypeptides related to ATF-43.Molecular and cellular biology · 1990Article
- Transcription factor Sp1 is important for retinoic acid-induced expression of the tissue plasminogen activator gene during F9 teratocarcinoma cell differentiation.Molecular and cellular biology · 1990Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
F9 cells induced to differentiate with retinoic acid (RA) increase transcription of the tissue plasminogen activator (t-PA) gene. Further treatment of these cells with cyclic AMP (cAMP) results in an additional stimulation of t-PA gene transcription. To investigate the mechanism of this two-stage regulation, 4 kilobase pairs (kbp) of 5'-flanking sequence from the murine t-PA gene was isolated. Two major start sites for transcription were found, neither of which depended on a classical TATA motif for correct initiation. By using transient transfection assays, it was determined that 4-kbp of flanking sequence could confer on reporter genes the same two-stage differentiation-specific expression as was observed for the endogenous t-PA gene. Deletion analyses of this 4-kbp fragment showed that 190 bp of flanking sequence was sufficient to bestow the same degree of two-stage regulation on reporter gene constructs. Within this region of DNA, sequence analysis revealed a possible cAMP regulatory element, a CTF/NF-1 recognition sequence, two potential Sp1 sites, and five potential binding sites for transcription factor AP-2. The deletion experiments, coupled with the positions of these potential cis-acting elements, suggest that multiple transcription factors, including those that bind to cAMP regulatory element, CTF/NF-1, Sp1, and AP-2 sites, may be involved in regulation of the t-PA gene during F9 cell differentiation.
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