Evidence map›Paper›PMID 25427613›Full record

ArticleGene therapy2015

AAV-mediated and pharmacological induction of Hsp70 expression stimulates survival of retinal ganglion cells following axonal injury.

J M K Kwong, L Gu, N Nassiri, V Bekerman, R Kumar-Singh, K D Rhee, X-J Yang, W W Hauswirth, J Caprioli, N Piri

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gene therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Strategies to Promote Long-Distance Optic Nerve Regeneration.Frontiers in cellular neuroscience · 2020
    Article
  8. Article
  9. Gene Therapy Strategies to Restore ER Proteostasis in Disease.Molecular therapy : the journal of the American Society of Gene Therapy · 2018
    Review
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

J M K KwongJules Stein Eye Institute, UCLA, Los Angeles, CA, USA.
L GuJules Stein Eye Institute, UCLA, Los Angeles, CA, USA.
N NassiriJules Stein Eye Institute, UCLA, Los Angeles, CA, USA.
V BekermanJules Stein Eye Institute, UCLA, Los Angeles, CA, USA.
R Kumar-SinghDepartment of Ophthalmology, Tufts University School of Medicine, Boston, MA, USA.
K D RheeJules Stein Eye Institute, UCLA, Los Angeles, CA, USA.
X-J YangJules Stein Eye Institute, UCLA, Los Angeles, CA, USA.
W W HauswirthDepartment of Ophthalmology, University of Florida, Gainesville, FL, USA.
J CaprioliJules Stein Eye Institute, UCLA, Los Angeles, CA, USA.
N PiriJules Stein Eye Institute, UCLA, Los Angeles, CA, USA.
Tufts University · USUniversity of Florida · US

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Gregory Darin Field, DAVID S WILLIAMS · 1985 to 2026
$16.8M
Translational Research for Retinal Degeneration TherapiesR01EY017549 · NEI · UNIVERSITY OF PENNSYLVANIA · PI BELTRAN, WILLIAM A. · 2007 to 2024
$14.1M
Vision Research CoreP30EY021721 · NEI · UNIVERSITY OF FLORIDA · PI HAUSWIRTH, WILLIAM W · 2011 to 2015
$2.8M
The Neuroprotective Effect of HSP72 Induction in Experimental GlaucomaR01EY018644 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PIRI, NATIK · 2009 to 2013
$1.3M
NEI NIH HHS EY018644NEI NIH HHS P30 EY000331NEI NIH HHS P30 EY021721NEI NIH HHS P30EY021721NEI NIH HHS R01 EY017549NEI NIH HHS R01 EY018644NEI NIH HHS R01EY17549
6 · The paper itself

Abstract

We evaluated the effect of AAV2- and 17-AAG (17-N-allylamino-17-demethoxygeldanamycin)-mediated upregulation of Hsp70 expression on the survival of retinal ganglion cells (RGCs) injured by optic nerve crush (ONC). AAV2-Hsp70 expression in the retina was primarily observed in the ganglion cell layer. Approximately 75% of all transfected cells were RGCs. RGC survival in AAV2-Hsp70-injected animals was increased by an average of 110% 2 weeks after the axonal injury compared with the control. The increase in cell numbers was not even across the retinas with a maximum effect of approximately 306% observed in the inferior quadrant. 17-AAG-mediated induction of Hsp70 expression has been associated with cell protection in various models of neurodegenerative diseases. We show here that a single intravitreal injection of 17-AAG (0.2 ug ul(-1)) results in an increased survival of ONC-injured RGCs by approximately 49% compared with the vehicle-treated animals. Expression of Hsp70 in retinas of 17-AAG-treated animals was upregulated approximately by twofold compared with control animals. Our data support the idea that the upregulation of Hsp70 has a beneficial effect on the survival of injured RGCs, and the induction of this protein could be viewed as a potential neuroprotective strategy for optic neuropathies.

Indexed as

AnimalsAxonsBenzoquinonesCell SurvivalCombined Modality TherapyDependovirusGenetic TherapyHSP70 Heat-Shock ProteinsHumansLactams, MacrocyclicMice, Inbred C57BLNerve CrushNerve RegenerationOptic Nerve InjuriesRetinaRetinal Ganglion CellsBenzoquinonesHSP70 Heat-Shock ProteinsLactams, Macrocyclictanespimycin

Identifiers

PMID25427613
PMCPMC4320032
OpenAlexW2323702396

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.