ArticlePloS one2014
Physiological and pathological impact of blood sampling by retro-bulbar sinus puncture and facial vein phlebotomy in laboratory mice.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
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- Safety and efficacy of iron supplementation after myocardial infarction in mice with moderate blood loss anaemia.ESC heart failure · 2021Article
- Comparison of murine retroorbital plexus and facial vein blood collection to mitigate animal ethics issues.Laboratory animal research · 2021Article
- Chemoprotective effects of inositol hexaphosphate against cyclophosphamide-induced testicular damage in rats.Scientific reports · 2020Article
- Anaemia is associated with severe RBC dysfunction and a reduced circulating NO pool: vascular and cardiac eNOS are crucial for the adaptation to anaemia.Basic research in cardiology · 2020Article
- The Impact of Common Recovery Blood Sampling Methods, in Mice (Mus Musculus), on Well-Being and Sample Quality: A Systematic Review.Animals : an open access journal from MDPI · 2020Review
- Impact of three commonly used blood sampling techniques on the welfare of laboratory mice: Taking the animal's perspective.PloS one · 2020Article
- Time-dependent Pathologic and Inflammatory Consequences of Various Blood Sampling Techniques in Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- Comparison of Serial Blood Collection by Facial Vein and Retrobulbar Methods in C57BL/6 Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2018Article
- Comparing Phlebotomy by Tail Tip Amputation, Facial Vein Puncture, and Tail Vein Incision in C57BL/6 Mice by Using Physiologic and Behavioral Metrics of Pain and Distress.Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Combined Deletion of Slc30a7 and Slc30a8 Unmasks a Critical Role for ZnT8 in Glucose-Stimulated Insulin Secretion.Endocrinology · 2016Article
- Evaluation of the appropriateness of using glucometers for measuring the blood glucose levels in mice.Scientific reports · 2016Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retro-bulbar sinus puncture and facial vein phlebotomy are two widely used methods for blood sampling in laboratory mice. However, the animal welfare implications associated with these techniques are currently debated, and the possible physiological and pathological implications of blood sampling using these methods have been sparsely investigated. Therefore, this study was conducted to assess and compare the impacts of blood sampling by retro-bulbar sinus puncture and facial vein phlebotomy. Blood was obtained from either the retro-bulbar sinus or the facial vein from male C57BL/6J mice at two time points, and the samples were analyzed for plasma corticosterone. Body weights were measured at the day of blood sampling and the day after blood sampling, and the food consumption was recorded automatically during the 24 hours post-procedure. At the end of study, cheeks and orbital regions were collected for histopathological analysis to assess the degree of tissue trauma. Mice subjected to facial vein phlebotomy had significantly elevated plasma corticosterone levels at both time points in contrast to mice subjected to retro-bulbar sinus puncture, which did not. Both groups of sampled mice lost weight following blood sampling, but the body weight loss was higher in mice subjected to facial vein phlebotomy. The food consumption was not significantly different between the two groups. At gross necropsy, subcutaneous hematomas were found in both groups and the histopathological analyses revealed extensive tissue trauma after both facial vein phlebotomy and retro-bulbar sinus puncture. This study demonstrates that both blood sampling methods have a considerable impact on the animals' physiological condition, which should be considered whenever blood samples are obtained.
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