ArticlePlastic and reconstructive surgery. Global open2014
Detergent-free Decellularized Nerve Grafts for Long-gap Peripheral Nerve Reconstruction.
Article in Plastic and reconstructive surgery. Global open, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- The effectiveness of acellular nerve allografts compared to autografts in animal models: A systematic review and meta-analysis.PloS one · 2024Pooled it
- Application strategies of autologous and decellularized nerve grafts: Structural and functional recovery.Neural regeneration research · 2026Article
- Peripheral Nerve Decellularisation Protocol for Allogeneic Transplantation: From Tissue Procurement to Banking.International journal of molecular sciences · 2025Article
- Preliminary study on the preparation of lyophilized acellular nerve scaffold complexes from rabbit sciatic nerves with human umbilical cord mesenchymal stem cells.World journal of stem cells · 2024Article
- Limited Nerve Regeneration across Acellular Nerve Allografts (ANAs) Coincides with Changes in Blood Vessel Morphology and the Development of a Pro-Inflammatory Microenvironment.International journal of molecular sciences · 2024Article
- Silk-in-Silk Nerve Guidance Conduits Enhance Regeneration in a Rat Sciatic Nerve Injury Model.Advanced healthcare materials · 2023Article
- Physical processing for decellularized nerve xenograft in peripheral nerve regeneration.Frontiers in bioengineering and biotechnology · 2023Review
- Effective decellularization of human nerve matrix for regenerative medicine with a novel protocol.Cell and tissue research · 2021Article
- Advances in the repair of segmental nerve injuries and trends in reconstruction.Muscle & nerve · 2020Review
- Decellularization techniques and their applications for the repair and regeneration of the nervous system.Methods (San Diego, Calif.) · 2020Review
- Characterizing Longitudinal Changes in the Impedance Spectra of In-Vivo Peripheral Nerve Electrodes.Micromachines · 2018Article
- Development of an apoptosis-assisted decellularization method for maximal preservation of nerve tissue structure.Acta biomaterialia · 2018Article
- Nerve Repair Using Decellularized Nerve Grafts in Rat Models. A Review of the Literature.Frontiers in cellular neuroscience · 2018Review
- Rodent model for assessing the long term safety and performance of peripheral nerve recording electrodes.Journal of neural engineering · 2017Article
- Developing Extracellular Matrix Technology to Treat Retinal or Optic Nerve Injury(1,2,3).eNeuro · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundLong-gap peripheral nerve defects arising from tumor, trauma, or birth-related injuries requiring nerve reconstruction are currently treated using nerve autografts and nerve allografts. Autografts are associated with limited supply and donor-site morbidity. Allografts require administration of transient immunosuppressants, which has substantial associated risks. To overcome these limitations, we investigated the use of detergent-free decellularized nerve grafts to reconstruct long-gap nerve defects in a rodent model and compared it with existing detergent processing techniques.
methodsNerve grafts were harvested from the sciatic nerves of 9 donor rats. Twenty-four recipient rats were divided into 4 groups (6 animals per group): (1) nerve grafts (NG, positive control), (2) detergent-free decellularized (DFD) grafts, (3) detergent decellularized grafts, and (4) silicone tube conduits (negative control). Each recipient rat had a 3.5-cm graft or conduit sutured across a sciatic nerve transection injury. All animals were harvested at 12 weeks postimplantation for functional muscle analysis and nerve histomorphometry.
resultsHistomorphometry results indicated maximum growth in NG when compared with other groups. DFD and detergent decellularized groups showed comparable regeneration at 12 weeks. Silicone tube group showed no regeneration as expected. Muscle force data indicated functional recovery in NG and DFD groups only.
conclusionsThis study describes a detergent-free nerve decellularization technique for reconstruction of long-gap nerve injuries. We compared DFD grafts with an established detergent processing technique and found that DFD nerve grafts are successful in promoting regeneration across long-gap peripheral nerve defects as an alternative to existing strategies.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.