ReviewFrontiers in plant science2014
Progress of cereal transformation technology mediated by Agrobacterium tumefaciens.
Review in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 159 citations in OpenAlex.
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- Molecular Regulators of In Vitro Regeneration in Wheat: Roles of Morphogenic Factors in Transformation, Genome Editing, and Breeding.International journal of molecular sciences · 2026Review
- Creation of TGMS Lines of Waxy Rice with Elite Physicochemical Properties of Starch via Waxy Gene Editing.Foods (Basel, Switzerland) · 2025Article
- CRISPR/Cas-mediated genome editing: playing a versatile role in mitigating the challenges of sustainable rice improvement.3 Biotech · 2025Review
- Article
- OsSDG715, a Histone H3K9 Methyltransferase, Integrates Auxin and Cytokinin Signaling to Regulate Callus Formation in Rice.Rice (New York, N.Y.) · 2025Article
- Developing Striga resistance in sorghum by modulating host cues through CRISPR/Cas9 gene editing.Plant cell reports · 2025Article
- Transcriptome Analysis Reveals the Pivotal Genes and Regulation Pathways Under Cold Stress and IdentifiesPlants (Basel, Switzerland) · 2025Article
- Unlocking regeneration potential: harnessing morphogenic regulators and small peptides for enhanced plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Stable transformation mediated byFrontiers in plant science · 2025Article
- Nuclear integration of MYB36 and APX-1 genes impart heat tolerance in wheat.Functional & integrative genomics · 2024Article
- Agrobacterium tumefaciens-Mediated Plant Transformation: A Review.Molecular biotechnology · 2024Review
- Enhancing wheat regeneration and genetic transformation through overexpression of TaLAX1.Plant communications · 2024Article
- Application of Developmental Regulators for Enhancing Plant Regeneration and Genetic Transformation.Plants (Basel, Switzerland) · 2024Review
- Establishment of a Rapid and EffectivePlants (Basel, Switzerland) · 2023Article
- An Efficient Transformation System for Fast Production ofPlants (Basel, Switzerland) · 2023Article
- Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.International journal of molecular sciences · 2023Review
- Risk assessment and bioburden evaluation of Agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter.BMC biotechnology · 2023Article
- Uncovering the transcriptional regulatory network involved in boosting wheat regeneration and transformation.Nature plants · 2023Article
- Whole-genome sequence analysis of mutations in rice plants regenerated from zygotes, mature embryos, and immature embryos.Breeding science · 2023Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monocotyledonous plants were believed to be not transformable by the soil bacterium Agrobacterium tumefaciens until two decades ago, although convenient protocols for infection of leaf disks and subsequent regeneration of transgenic plants had been well established in a number of dicotyledonous species by then. This belief was reinforced by the fact that monocotyledons are mostly outside the host range of crown gall disease caused by the bacterium and by the failures in trials in monocotyledons to mimic the transformation protocols for dicotyledons. However, a key reason for the failure could have been the lack of active cell divisions at the wound sites in monocotyledons. The complexity and narrow optimal windows of critical factors, such as genotypes of plants, conditions of the plants from which explants are prepared, tissue culture methods and culture media, pre-treatments of explants, strains of A. tumefaciens, inducers of virulence genes, transformation vectors, selection marker genes and selective agents, kept technical hurdles high. Eventually it was demonstrated that rice and maize could be transformed by co-cultivating cells of callus cultures or immature embryos, which are actively dividing or about to divide, with A. tumefaciens. Subsequently, these initial difficulties were resolved one by one by many research groups, and the major cereals are now transformed quite efficiently. As many as 15 independent transgenic events may be regenerated from a single piece of immature embryo of rice. Maize transformation protocols are well established, and almost all transgenic events deregulated for commercialization after 2003 were generated by Agrobacterium-mediated transformation. Wheat, barley, and sorghum are also among those plants that can be efficiently transformed by A. tumefaciens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.