ArticleFrontiers in molecular neuroscience2014
Defining the nociceptor transcriptome.
Article in Frontiers in molecular neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed.
- A review of preclinical research on mechanically gated ion channels as therapeutic targets in neuropathic pain.Annals of medicine · 2026Review
- Heart-Specific Spinal and Vagal Afferents: Transcriptomic Signatures and Optogenetically Modulated Functional Coupling With Cardiomyocytes.Comprehensive Physiology · 2026Article
- Human pain transcriptomics: lessons learned so far.Pain reports · 2026Review
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- A neuron type-specific microexon in Ank3/ankyrin-G modulates calcium activity and neuronal excitability.Nature communications · 2026Article
- A neuron type-specific microexon inbioRxiv : the preprint server for biology · 2025Article
- Sex-specific involvement of calcitonin gene-related peptide signaling for pain in experimental autoimmune encephalomyelitis.Pain reports · 2025Article
- IL-17 Stimulates Sensory Neurons and Sensitises Colonic Afferents to Noxious Stimuli in a PI3K Dependent Manner in Male Mice.Journal of neurochemistry · 2025Article
- Modelling inflammation-induced peripheral sensitization in a dish-more complex than expected?Pain · 2025Article
- Renal interoception in health and disease.Autonomic neuroscience : basic & clinical · 2024Review
- Phosphatidic acid is an endogenous negative regulator of PIEZO2 channels and mechanical sensitivity.Nature communications · 2024Article
- IL-1R Mediated Activation of Renal Sensory Nerves in DOCA-Salt Hypertension.Hypertension (Dallas, Tex. : 1979) · 2024Article
- Satellite Glial Cells Bridge Sensory Neuron Crosstalk in Visceral Pain and Cross-Organ Sensitization.The Journal of pharmacology and experimental therapeutics · 2024Review
- Neuroimmune Interactions in the Intestine.Annual review of immunology · 2024Review
- Phosphatidic acid is an endogenous negative regulator of PIEZO2 channels and mechanical sensitivity.bioRxiv : the preprint server for biology · 2024Article
- Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
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- A zebrafish model of Ifih1-driven Aicardi-Goutières syndrome reproduces the interferon signature and the exacerbated inflammation of patients.Frontiers in immunology · 2023Article
- Type 2 cytokines sensitize human sensory neurons to itch-associated stimuli.Frontiers in molecular neuroscience · 2023Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
Funding
Abstract
Unbiased "omics" techniques, such as next generation RNA-sequencing, can provide entirely novel insights into biological systems. However, cellular heterogeneity presents a significant barrier to analysis and interpretation of these datasets. The neurons of the dorsal root ganglia (DRG) are an important model for studies of neuronal injury, regeneration and pain. The majority of investigators utilize a dissociated preparation of whole ganglia when studying cellular and molecular function. We demonstrate that the standard methods for producing these preparations gives a 10%-neuronal mixture of cells, with the remainder of cells constituting satellite glia and other non-neuronal cell types. Using a novel application of magnetic purification, we consistently obtain over 95% pure, viable neurons from adult tissue, significantly enriched for small diameter nociceptors expressing the voltage gated ion channel Nav1.8. Using genome-wide RNA-sequencing we compare the currently used (10% neuronal) and pure (95% nociceptor) preparations and find 920 genes enriched. This gives an unprecedented insight into the molecular composition of small nociceptive neurons in the DRG, potentially altering the interpretation of previous studies performed at the tissue level, and indicating a number of novel markers of this widely-studied population of cells. We anticipate that the ease of use, affordability and speed of this technique will see it become widely adopted, delivering a greatly improved capacity to study the roles of nociceptors in health and disease.
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